The current study was conducted to evaluate the different concentrations of deltamethrin (DEL; Exp. 1) and the protective role of idebenone (IDB) supplemented with toxic dose of deltamethrin (Exp. 2) during chilled storage of ram semen. Collected samples were pooled and diluted at 500×10⁶ spermatozoa per mL. In Exp. 1, effect of DEL at 1, 2.5, 5, 10, 20, 40, 80 μM levels were evaluated on different variables of spermatozoa motion characteristics. In Exp. 2, different amounts of IDB (2, 4, and 8 μM) concurrent with constant doses of DEL (10 μM) were examined on semen quality upon chilled preservation up to 72 h. Indices of spermatozoa kinematics, functionality of plasma membrane and viability were recorded. Biochemical metabolites were measured in spermatozoa and its medium (extender) at different time points. In Exp.1, different parameters of spermatozoa kinematics were affected by exposure to DEL in a dose dependent manner. In Exp. 2, combination of IDB with DEL, resulted in a significant (P < 0.05) increase in total motility, forward progressive motility and curvilinear path velocity compared to DEL group at 24, 48 and 72 h. Simultaneous administration of IDB with DEL increased the percentage of spermatozoa with functional membrane and viability compared to DEL group at 72 h (P < 0.05). The amounts of lipid peroxidation index were lower in medium of combination groups compared to DEL group at 48 h and 72 h (P < 0.05). Antioxidant capacity were higher in spermatozoa and medium of IDB treated groups compared to DEL group at 72 h (P < 0.05). Amounts of total nitratenitrite and superoxide dismutase activity of spermatozoa and medium did not affect by treatment (P > 0.05). In conclusion, IDB could ameliorate oxidative and peroxidative damages induced by DEL mild toxicity upon cold preservation of ram semen.

中文翻译：

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艾地苯醌对溴氰菊酯介导的4°C下精液过氧化毒性的减弱作用

进行当前的研究以评估不同浓度的溴氰菊酯（DEL。Exp.1）和艾地苯醌（IDB）补充有毒剂量的溴氰菊酯（Exp。2）在冷藏精液中的保护作用。收集样品并在500×10 ⁶稀释每毫升精子。在实验中 如图1所示，在精子运动特性的不同变量上评估了DEL在1、2.5、5、10、20、40、80μM水平下的作用。在实验中 如图2所示，在冷藏保存长达72小时后，检查了不同量的IDB（2、4和8μM）以及恒定剂量的DEL（10μM）对精液质量的影响。记录精子运动学指标，质膜功能和生存力。在不同的时间点在精子及其培养基（补充剂）中测量生化代谢产物。在实验1中，精子运动学的不同参数受到剂量依赖性暴露于DEL的影响。在实验中 如图2所示，与DEL组相比，在24、48和72 h时，IDB与DEL的组合导致总运动，向前进行性运动和曲线路径速度显着（P <0.05）增加。与DEL组相比，IDB的IDB同时给药增加了72 h时具有功能膜和活力的精子百分比（P <0.05）。与DEL组相比，在48 h和72 h时，联合治疗组的脂质过氧化指数降低（P <0.05）。与DEL组相比，IDB治疗组在72 h时的抗氧化能力更高（P <0.05）。精子和培养基中总亚硝酸盐和超氧化物歧化酶的活性不受处理的影响（P> 0.05）。总之，IDB可以减轻DEL轻度毒性对公羊精液冷藏后诱导的氧化和过氧化损伤。