The objective of this study was to investigate the effect of diets containing different levels of dried tuber of Jerusalem artichoke, Helianthus tuberosus, on skatole levels in back fat and on the CYP2E1 mRNA expression in the liver of commercial crossbred pigs. A total of 23 uncastrated male pigs from 10 litters of a commercial crossbred population of Large White × (Landrace × Large White), were used in this study. Boars were randomly divided into four different dietary treatment groups - a control group (K1; 5 boars; without supplementation of Jerusalem artichoke,) and three experimental groups (6 boars each) that were fed with the diet containing different levels of dried Jerusalem artichoke (K2 – 4.1%; K3 – 8.2%; K4 – 12.2%) for 14 days before slaughter. Significant effects of diet on skatole levels were observed between the control group and the experimental groups (P = 0.0078). The lowest level of skatole was in the K3 group with 8.2% of Jerusalem artichoke. As for CYP2E1, a negative correlation was observed between the levels of skatole and CYP2E1 mRNA expression. Significant effect (P = 0.0055) was found in all experimental groups compared to the K1 group, and most pronounced in the K2 and K3 groups. The supplementation with Jerusalem artichoke resulted in lower level of skatole and higher CYP2E1 mRNA expression. The results suggest that affecting the expression of CYP2E1 by feed supplements could be an option to effectively reduce the levels of skatole in adipose tissue of entire male pigs.

中文翻译：

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日粮菊芋干燥块茎对野猪粪便中粪臭素水平和CYP2E1 mRNA表达的影响。

这项研究的目的是调查含有不同程度的菊芋，干燥块茎饲料的效果菊芋，在背部脂肪和粪臭素的水平CYP2E1商品杂交猪肝脏中的mRNA表达。这项研究使用了来自商业杂种大白×（大白×大白）的10窝的23头未cast割的公猪。将公猪随机分为四个不同的饮食治疗组-对照组（K1; 5个公猪;不补充菊芋）和三个实验组（每个6个公猪）饲喂含不同水平的菊芋干的饮食（ K2 – 4.1％； K3 – 8.2％； K4 – 12.2％），屠宰前14天。在对照组和实验组之间，饮食对粪臭素水平具有显着影响（P = 0.0078）。粪臭素水平最低的是K3组，占菊芋的8.2％。至于CYP2E1，在粪臭素水平和CYP2E1 mRNA表达之间观察到负相关。与K1组相比，在所有实验组中均发现了显着效果（P = 0.0055），在K2和K3组中最为明显。补充洋姜导致较低的粪臭素水平和较高的CYP2E1 mRNA表达。结果表明，通过饲料添加剂影响CYP2E1的表达可能是有效降低整个雄性猪脂肪组织中粪臭素水平的一种选择。