Plants produce a huge number of functionally and chemically different natural products that play an important role in linking the plant with the adjacent environment. Plants can also absorb and transform external organic compounds (xenobiotics). Currently there are only a few studies concerning the effects of xenobiotics and their transformation products on plant metabolites using a mass spectrometric untargeted screening strategy. This study was designed to investigate the changes of the Phragmites australis metabolome following/after diclofenac or carbamazepine incubation, using a serial coupling of reversed-phase liquid chromatography (RPLC) and hydrophilic interaction liquid chromatography (HILIC) combined with accurate high-resolution time-of-flight mass spectrometer (TOF-MS). An untargeted screening strategy of metabolic fingerprints was developed to purposefully compare samples from differently treated P. australis plants, revealing that P. australis responded to each drug differently. When solvents with significantly different polarities were used, the metabolic profiles of P. australis were found to change significantly. For instance, the production of polyphenols (such as quercetin) in the plant increased after diclofenac incubation. Moreover, the pathway of unsaturated organic acids became more prominent, eventually as a reaction to protect the cells against reactive oxygen species (ROS). Hence, P. australis exhibited an adaptive mechanism to cope with each drug. Consequently, the untargeted screening approach is essential for understanding the complex response of plants to xenobiotics.

中文翻译：

---

药物培养的芦苇轮廓的非靶向代谢组学研究

植物产生大量功能上和化学上不同的天然产物，这些产物在将植物与邻近环境联系起来方面起着重要作用。植物还可以吸收和转化外部有机化合物（异质生物）。目前，仅有少数几项关于异生物素及其转化产物对植物代谢产物的影响的研究，采用的是质谱无目标筛选策略。本研究旨在调查芦苇的变化双氯芬酸或卡马西平孵育后/之后的代谢组，使用反相液相色谱（RPLC）和亲水相互作用液相色谱（HILIC）的串联偶联结合精确的高分辨率飞行时间质谱仪（TOF-MS）。开发了一种无目标的代谢指纹图谱筛选策略，目的是有目的地比较来自不同处理的澳大利亚假单胞菌植物的样品，从而揭示澳大利亚假单胞菌对每种药物的反应不同。当使用极性显着不同的溶剂时，澳大利亚假单胞菌的代谢谱被发现有很大变化。例如，双氯芬酸孵育后植物中多酚（例如槲皮素）的产量增加。此外，不饱和有机酸的途径变得更加突出，最终成为保护细胞免受活性氧（ROS）侵害的反应。因此，澳大利亚假单胞菌表现出适应每种药物的适应性机制。因此，非靶向筛选方法对于理解植物对异种生物的复杂反应至关重要。