Abstract

This study evaluated how adding EGCG to a SEP (Clearfil SE Bond, Kuraray) affects the physicomechanical properties of adhesive, and the long-term microtensile bond strength (µTBS) to dentin. Flexural strength (FS, n = 10) and degree of conversion (DC, n = 5) of the adhesive were analyzed after adding EGCG to SEP in two forms (powder or diluted in water), and in different concentrations (0.00%–control, 0.01%, 0.02% and 0.05%). The µTBS was tested by randomly dividing dentin fragments into 4 groups (n = 10): EGCG-SEP: EGCG (0.01%) added to SEP; EGCG-PRE: EGCG (0.01%) dentin pretreatment; CHX-PRE: 2% chlorhexidine dentin pretreatment; SEP: SEP applied without modification. After restoration of the dentin surface, beam-shaped specimens were submitted to µTBS after 24 h or 12 months of storage in artificial saliva. Particle size (PS), polydispersity index (PDI) and zeta potential (ZP) of EGCG-SEP and SEP solutions were measured at different storage periods (baseline, 14 or 28 days). Data was submitted to ANOVA or Kruskal–Wallis tests (α = 0.05). FS (p = 0.485) and DC (p = 0.6067) were not different among the groups. CHX-PRE and EGCG-PRE had significantly higher µTBS means than EGCG-SEP, but none differed from SEP (p = 0.053). µTBS decreased significantly after 12 months of storage (p < 0.001). PS (p < 0.001), and ZP (p = 0.003) means in EGCG-SEP were statistically higher and more negative, respectively, than SEP. PDI did not differ among the groups (p > 0.05). Incorporation of EGCG into SEP did not change adhesive properties; however, it influenced solution stability and did not prevent the decrease in long-term µTBS to dentin.

摘要

本研究评估了将 EGCG 添加到 SEP（Clearfil SE Bond，Kuraray）中如何影响粘合剂的物理机械特性以及与牙本质的长期微拉伸粘合强度 (μTBS)。弯曲强度（FS，Ñ  = 10）和转化度（DC，Ñ 粘合剂= 5）以两种形式加入到EGCG后SEP分析（粉末或在水中稀释），并在不同浓度（0.00％ -控制、0.01%、0.02% 和 0.05%）。通过将牙本质碎片随机分为 4 组（n = 10)：EGCG-SEP：EGCG (0.01%) 添加到 SEP；EGCG-PRE：EGCG（0.01%）牙本质预处理；CHX-PRE：2%氯己定牙本质预处理；SEP：SEP 未经修改即适用。牙本质表面修复后，梁形标本在人工唾液中储存 24 小时或 12 个月后提交给 μTBS。EGCG-SEP 和 SEP 溶液的粒径 (PS)、多分散指数 (PDI) 和 zeta 电位 (ZP) 在不同的储存期（基线、14 或 28 天）进行测量。数据提交到方差分析或 Kruskal-Wallis 检验 (α = 0.05)。FS ( p  = 0.485) 和 DC ( p  = 0.6067) 在各组之间没有差异。CHX-PRE 和 EGCG-PRE 的 µTBS 均值显着高于 EGCG-SEP，但与 SEP 均无差异（p = 0.053）。储存 12 个月后，µTBS 显着下降 ( p  < 0.001)。 EGCG-SEP 中的PS ( p  < 0.001) 和 ZP ( p = 0.003) 均值分别高于 SEP 和更负值。PDI 在各组之间没有差异（p  > 0.05）。在 SEP 中加入 EGCG 不会改变粘合性能；然而，它影响了溶液的稳定性，并不能阻止长期 µTBS 对牙本质的减少。