Abstract Biofilms were developed from human saliva on bovine enamel discs in four experimental conditions to investigate dental caries development: feast and famine (M1), abundance and scarcity (M2), three meals daily (M3), and three meals plus two snacks daily (M4). The main difference between these models was the diet for microbial growth. The evaluations included verifying the pH of the spent culture media and analyzing the enamel discs for demineralization (microhardness and roughness) and biofilms (biomass, viable populations of mutans streptococci, and total microbiota). Two major behaviors were observed: M1 and M2 promoted an acidic environment, while M3 and M4 maintained pH values closer to neutral. The demineralization process was slower in the neutral groups but more pronounced in M3, while a greater increase in microbiota and biomass was observed over time for both neutral groups. Thus, the M3 model was better at mimicking the oral environment that leads to demineralization.

中文翻译：

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再现致龋饮食摄入量的微观生物膜模型的建立

摘要 在四种实验条件下，在牛牙釉质盘上从人类唾液中形成生物膜以研究龋齿的发展：盛宴和饥荒（M1）、丰富和稀缺（M2）、每日三餐（M3）和每日三餐加两餐。 M4）。这些模型之间的主要区别在于微生物生长的饮食。评估包括验证用过的培养基的 pH 值并分析搪瓷盘的脱矿质（显微硬度和粗糙度）和生物膜（生物量、变形链球菌的可行种群和总微生物群）。观察到两种主要行为：M1 和 M2 促进酸性环境，而 M3 和 M4 保持更接近中性的 pH 值。中性组的脱矿过程较慢，但在 M3 中更为明显，而随着时间的推移，两个中性组都观察到微生物群和生物量的更大增加。因此，M3 模型更擅长模仿导致脱矿质的口腔环境。