Four new β-resorcylic acid lactones, including penochrochlactone A (2: ), 4-O-desmethyl-aigialomycin B (4: ), and penochrochlactones C and D (5: and 6: ), two compounds isolated from a natural source for the first time, 5α, 6β-acetonide-aigialomycin B (1: ) and penochrochlactone B (3: ), together with six known compounds, aigialomycin F (7: ), aigialomycins A, B, and D (8: -10: ), zeaenol (11: ), and oxozeaenol (12: ), were isolated from a mycelial solid culture of the endophytic fungus Penicillium ochrochloron SWUKD4.1850 from the medicinal plant Kadsura angustifolia by sequential purification over silica gel, Sephadex LH-20 column chromatography, and preparative HPLC. Their structures were elucidated by extensive spectroscopic analysis and chemical conversions. In addition, all the new compounds were evaluated for their cytotoxic and antibacterial activities in vitro. Penochrochlactone C (5: ) displayed moderate cytotoxicity against the HeLa tumor cell line with an IC50 value of 9.70 µM. In the antibacterial assays, compounds 4: - 6: exhibited moderate activities against Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Pseudomonas aeruginosa with MIC values between 9.7 and 32.0 µg/mL.

中文翻译：

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由 Kadsura angustifolia 内生青霉 ochrochloron 菌株产生的间苯二酸内酯

四种新的 β-间苯二酸内酯，包括苯甲酸内酯 A (2: )、4-O-去甲基-aigalomycin B (4: ) 和苯甲酸内酯 C 和 D (5: 和 6: )，这两种化合物是从天然来源分离出来的第一次，5α, 6β-丙酮化物-aigialomycin B (1: ) 和青霉素 B (3: )，连同六种已知化合物，aigialomycin F (7: )，aigialomycins A、B 和 D (8: -10: )、玉米烯醇 (11: ) 和氧化玉米烯醇 (12: ) 是从药用植物 Kadsura angustifolia 的内生真菌 Penicillium ochrochloron SWUKD4.1850 的菌丝体固体培养物中分离出来的，通过硅胶顺序纯化，Sephadex LH-20 柱层析和制备型 HPLC。通过广泛的光谱分析和化学转化阐明了它们的结构。此外，对所有新化合物的体外细胞毒性和抗菌活性进行了评估。Penochrochlactone C (5: ) 对 HeLa 肿瘤细胞系显示出中等的细胞毒性，IC50 值为 9.70 µM。在抗菌试验中，化合物 4: - 6: 对金黄色葡萄球菌、枯草芽孢杆菌、大肠杆菌和铜绿假单胞菌表现出中等活性，MIC 值介于 9.7 和 32.0 µg/mL 之间。