BACKGROUND Hepatocellular carcinoma (HCC) is a major cause of cancer death in Egypt. There is still a risk for HCC development even after eradicating hepatitis C virus (HCV) by direct-acting antivirals (DAAs). Chitinase-3-like protein1(CHI3L1),a biomarker for predicting many diseases, plays an essential role in inflammation, angiogenesis, and antiapoptosis. Tolloid-like protein 1 (TLL1) may be involved in hepatic fibrogenesis and carcinogenesis. OBJECTIVES Determining the role and combined effect of CHI3L1 (rs880633), TLL1 (rs1503298), and an intergenic (rs597533) polymorphisms on the risk of developing HCC in Egyptian patients after achieving SVR by DAAs. SUBJECTS AND METHODS Blood samples were collected from 68 HCC patients, 77 non-HCC subjects and 80 healthy controls. The DNA was extracted and analyzed for rs880633, rs1503298 and rs597533 using Genotyping TaqManTM assay. RESULTS A significant difference in genotypes and alleles frequencies was observed in both (rs880633) and (rs597533) between HCC group as compared to healthy control and also as compared to the non-HCC group. However, regarding to (rs1503298) genotypes and alleles between the HCC and non-HCC groups, there were no significant differences. Combined polymorphism in more than one gene simultaneously showed a higher risk to HCC after SVR than an individual locus. CONCLUSION Both allelic and genotypic variations of the CHI3L1 gene (rs880633) and an intergenic (rs597533) seemed to be significant predictors confirming a great risk for HCC susceptibility in Egyptian patients achieved SVR. Patients with a polymorphism in more than one gene showed an increased risk to HCC after SVR rather than individual locus. This article is protected by copyright. All rights reserved.

中文翻译：

---

几丁质酶 3 样 1、Tolloid 样蛋白 1 和基因间基因多态性是直接作用抗病毒药物根除 HCV 后肝细胞癌发展的预测因子

背景技术肝细胞癌(HCC)是埃及癌症死亡的主要原因。即使在通过直接作用抗病毒药物 (DAA) 根除丙型肝炎病毒 (HCV) 后，仍存在发生 HCC 的风险。几丁质酶 3 样蛋白 1 (CHI3L1) 是预测许多疾病的生物标志物，在炎症、血管生成和抗细胞凋亡中起重要作用。Tolloid 样蛋白 1 (TLL1) 可能参与肝纤维化和癌变。目的 确定 CHI3L1 (rs880633)、TLL1 (rs1503298) 和基因间 (rs597533) 多态性对埃及患者通过 DAA 实现 SVR 后发生 HCC 风险的作用和综合作用。受试者和方法 血液样本采集自 68 名 HCC 患者、77 名非 HCC 受试者和 80 名健康对照者。提取 DNA 并分析 rs880633，rs1503298 和 rs597533 使用基因分型 TaqMan™ 分析。结果 与健康对照组和非HCC组相比，HCC组（rs880633）和（rs597533）的基因型和等位基因频率存在显着差异。然而，关于 (rs1503298) 基因型和等位基因，HCC 组和非 HCC 组之间没有显着差异。与单个基因座相比，多个基因的组合多态性同时显示 SVR 后发生 HCC 的风险更高。结论 CHI3L1 基因 (rs880633) 和基因间 (rs597533) 的等位基因和基因型变异似乎是证实埃及患者实现 SVR 的 HCC 易感性的巨大风险的重要预测因子。具有多个基因多态性的患者在 SVR 后显示出患 HCC 的风险增加，而不是单个基因座。本文受版权保护。版权所有。