Stem cell therapy promotes tissue regeneration and wound healing. Efforts have been made to prime stem cells to enhance their regenerative abilities. Certain marijuana components, namely the non‐psychoactive cannabidiol (CBD) and psychoactive tetrahydrocannabinol (THC), are defined as immunomodulators.⁹ We test whether two sources of stem cells, primed with CBD or THC, would demonstrate improved regenerative abilities. Human adipose‐derived stem cells (ASCs) and bone marrow‐derived stem cells (BMDSCs), not obtained from the same individual, were treated with low (300 nM) or high (3 μM) concentration CBD. Porcine ASCs and BMDSCs were isolated from a single pig, and treated with either low or high concentrations of CBD or THC. Transwell migration and MTT proliferation assays were performed on the human ASCs and BMDSCs. Also, transwell migration assay was performed on the porcine ASCs and BMDSCs. Finally, a wound healing scratch assay in porcine primary fibroblasts (PFs) was performed, co‐cultured with the cannabinoid‐treated ASCs. CBD priming at low concentration induces migration by 180% (P < .01) in porcine ASCs, and by only 93% (P < .02) in porcine BMDSCs. In porcine stem cells, THC priming at low concentration induces migration by 91.6% (P < .01) in ASCs but by only 44.3% (P < .03) in BMDSCs. Compared to PFs co‐cultured with untreated ASCs, PFs co‐cultured with low CBD‐primed ASCs had 75% faster wound closure at 18 hours (P < .01). CBD and THC priming of ASCs and BMDSCs, particularly at lower doses, enhances a number of regenerative parameters, suggesting that these major marijuana components may improve stem cell‐based therapies.

中文翻译：

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大麻成分对干细胞再生能力的作用

干细胞疗法可促进组织再生和伤口愈合。已经努力启动干细胞以增强其再生能力。某些大麻成分，即非精神活性大麻二酚（CBD）和精神活性四氢大麻酚（THC），被定义为免疫调节剂。⁹我们测试了用CBD或THC引发的两种干细胞来源是否会显示出改善的再生能力。并非从同一个体获得的人脂肪干细胞（ASC）和骨髓干细胞（BMDSC）分别用低浓度（300 nM）或高浓度（3μM）的CBD处理。从一头猪中分离出猪ASC和BMDSC，并用低或高浓度的CBD或THC处理。在人类ASC和BMDSC上进行了Transwell迁移和MTT增殖测定。另外，对猪ASC和BMDSC进行了跨孔迁移测定。最后，在猪原代成纤维细胞（PFs）中进行了伤口愈合刮擦试验，并与大麻素处理过的ASC共培养。低浓度的CBD启动引发迁移180％（P 猪ASC中的<0.01）和 猪BMDSC中的93％（P <.02）。在猪干细胞中，低浓度的THC引发 在ASCs中诱导迁移达91.6％（P <.01），而 在BMDSCs中仅诱导44.3％（P <.03）。与未经处理的ASC共培养的PF相比，与低CBD引发的ASC共培养的PF在18小时时伤口闭合的速度加快了75％（P  <.01）。ASC和BMDSC的CBD和THC引发，特别是在较低剂量下，可以增强许多再生参数，这表明这些主要的大麻成分可能会改善基于干细胞的疗法。