Interferon stimulated gene 15 (ISG15) is one of the most highly upregulated proteins in response to viral infection and is involved in numerous pathways with multiple mechanisms of actions. ISG15 deficiency has been reported to induce type I interferonopathy owing to defective negative regulation of IFN-I signalling as well as enhanced antiviral protection. Here, we have generated ISG15 knockout clones from human iPSCs, which provide useful cell resources to study mechanisms of ISG15 deficiency and gain more insight into the biological function of ISG15.

中文翻译：

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使用 CRISPR/Cas9 编辑生成两个人类 ISG15 敲除 iPSC 克隆

干扰素刺激基因 15 (ISG15) 是病毒感染反应中上调程度最高的蛋白质之一，参与多种具有多种作用机制的途径。据报道，ISG15 缺陷会由于 IFN-I 信号传导的负调节缺陷以及抗病毒保护的增强而诱发 I 型干扰素病。在这里，我们从人类 iPSC 中产生了 ISG15 敲除克隆，这为研究 ISG15 缺陷机制并更深入地了解 ISG15 的生物学功能提供了有用的细胞资源。