A ten-week feeding trial was conducted to investigate the effects of the supplementation of Clostridium autoethanogenum protein (CAP) in practical diets on the growth performance, serum chemical parameters, antioxidant status and histopathological examination of the liver and midgut in juvenile Jian carp. Five isnitrogenous and isenergetic diets were formulated with graded levels of CAP (0, 50, 100, 150 and 200 g/kg) to replace plant proteins. The results showed that, final body weight, percent weight gain, specific growth rate, protein retention value, protein efficiency ratio and condition factor were significantly improved in fish fed with CAP, while feed conversion ratio was the opposite(P < 0.05). Visceral somatic index was significantly decreased in the fish fed with CAP (P < 0.05). Serum total cholesterol and total triglyceride contents were significantly increased with increasing dietary CAP levels, and serum lactate dehydrogenase showed an opposite trend (P < 0.05). Meanwhile, aspartate aminotransferase activities were significantly decreased with increasing dietary CAP levels (P < 0.05). The serum activities of the catalase were significantly increased with an increase in CAP levels (P < 0.05); however, malondialdehyde contents showed the opposite trend (P < 0.05). The mRNA expression levels of growth-related genes (PepT-1, PepT-2 and IGF-1), Nrf2 signaling pathway-related genes (Nrf2, Cu/Zn-SOD, HO-1, GPx, Mn-SOD and CAT) and TOR signaling pathway-related genes (TOR, 4E-BP2 and S6K1) were improved by the addition of dietary CAP (P < 0.05). Furthermore, from histological examination, CAP had no obvious effect on the liver and midgut. The present results indicated that CAP supplementation in practical diets has a beneficial effect on growth performance and antioxidant capacity and does not have an obvious effect on the liver and midgut in juvenile Jian carp. This study has shown the potential of converting industrial waste into a high protein feed ingredient for aquafeeds.

进行了为期十周的喂养试验，以研究在实际饮食中补充梭菌梭状芽胞杆菌蛋白（CAP）对Jian鱼的生长性能，血清化学参数，抗氧化剂状态以及肝脏和中肠组织病理学检查的影响。配制了五种等氮和高能饮食，以CAP的分级水平（0、50、100、150和200 g / kg）代替植物蛋白。结果表明，饲喂CAP的鱼的最终体重，体重增加百分比，比生长速率，蛋白质保留值，蛋白质效率比和条件因子均得到显着改善，而饲料转化率则相反（P <0.05）。CAP喂养的鱼的内脏体细胞指数显着降低（P ＜0.05）。血清总胆固醇和总甘油三酯含量随日粮CAP水平的增加而显着增加，而血清乳酸脱氢酶则呈现相反的趋势（P <0.05）。同时，随着日粮CAP水平的提高，天冬氨酸转氨酶活性显着降低（P <0.05）。随着CAP水平的升高，过氧化氢酶的血清活性显着增加（P <0.05）; 然而，丙二醛含量却呈现相反的趋势（P <0.05）。生长相关基因（PepT-1，PepT-2和IGF-1），Nrf2信号通路相关基因（Nrf2，Cu / Zn-SOD，HO-1，GPx，Mn-SOD和CAT）的mRNA表达水平饮食CAP改善了TOR信号通路相关基因（TOR，4E-BP2和S6K1）（P <0.05）。此外，从组织学检查来看，CAP对肝脏和中肠无明显作用。目前的结果表明，在日常饮食中补充CAP对has鱼的生长性能和抗氧化能力具有有益的作用，对肝脏和中肠没有明显的作用。这项研究表明了将工业废物转化为水产饲料的高蛋白饲料成分的潜力。