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Screening and genetic studies on resistance to Soil-born Cereal Mosaic Virus (SBWMV) in rye
Cereal Research Communications ( IF 1.6 ) Pub Date : 2020-12-22 , DOI: 10.1007/s42976-020-00105-1
R. Schlegel , J. Eifler , M. Schmidt , B. Schmiedchen , F. Ordon , U. Kastirr

Due to several reasons soil-borne viruses such as the furoviruses, i. e., cereal mosaic virus (SBCMV) and wheat mosaic virus (SBWMV) as well as the bymovirus wheat spindle streak mosaic virus (WSSMV) gained importance in cereal breeding including rye. High yield losses are recorded, today. Since there is no or little resistance to these viruses in modern rye cultivars, an extended screening for resistance was initiated. In addition to earlier screenings, 37 rye genotypes were tested for resistance. Among them, three genotypes were found with persistent resistance to SBCMV. They belong to Secale montanum and S. vavilovii species, i. e., wild types of rye. One accession, PC2243 (S. montanum), was used as a resistance donor for the present genetic study. In F2 generation, it was observed that resistance to SBCMV is independently inherited from WSSMV. The evaluation of the ELISA values pointed to a 3:1 distribution assuming duplicate dominant epistasis. Molecular marker analysis supports this segregation pattern. By composite interval mapping a QTL on chromosome 2R could be detected. It can be assumed that there is a DNA region of about 13 cM on the long arm of chromosome 2R (2RL) harboring SBCMV resistance with the closest markers “C9654_1947” and “isotig11640”. Moreover, genotypes with a yellow seed coat showed practically no infection with SBCMV. Thus, the resistance gene could be linked to the allele an1 determining non expression of anthocyanins. This locus was also mapped earlier on chromosome 2R.



中文翻译:

黑麦对土壤中携带的谷物花叶病毒的抗性筛选和遗传研究

由于多种原因,土壤传播的病毒,例如呋喃病毒,即谷物花叶病毒(SBCMV)和小麦花叶病毒(SBWMV)以及副病毒小麦梭条花叶病毒(WSSMV)在包括黑麦在内的谷物育种中变得越来越重要。今天记录到高产损失。由于在现代黑麦品种中对这些病毒几乎没有抗药性,因此开始了对抗药性的扩展筛选。除了较早的筛选,还测试了37种黑麦基因型的抗性。其中,发现了对SBCMV具有持久抗性的三种基因型。它们属于Secale montanumS. vavilovii物种,即黑麦的野生类型。一种登录号PC2243(S. montanum)用作本基因研究的抗性供体。在F2代中,观察到对SBCMV的抗性独立于WSSMV。ELISA值的评估结果表明,假设存在重复的显性上位性,则为3:1分布。分子标记分析支持这种分离模式。通过复合间隔图谱,可以检测到染色体2R上的QTL。可以假设在染色体2R(2RL)的长臂上存在一个约13 cM的DNA区域,带有SBCMV抗性,带有最接近的标记“ C9654_1947”和“ isotig11640”。此外,带有黄色种皮的基因型几乎没有显示出SBCMV感染。因此,抗性基因可以与等位基因an1连锁确定花青素的不表达。该基因座也较早地定位在染色体2R上。

更新日期:2020-12-22
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