The cell is the unit of life for all organisms, and all cells are certainly not the same. So the technology to generate transcription expression or genomic DNA profiles from single cells is crucial. Since its establishment in 2009, single-cell RNA sequencing (scRNA-seq) has emerged as a major driver of progress in biomedical research. During the last three years, several new single-cell sequencing platforms have emerged. Yet there are only a few systematic comparisons of the advantages and limitations of these commonly used platforms. Here we compare two single-cell sequencing platforms: BD Rhapsody and 10x Genomics Chromium, including their different mechanisms and some scRNA-seq results obtained with them.

中文翻译：

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两种单细胞测序平台的比较：BD Rhapsody 和 10x Genomics Chrominum

细胞是所有生物体的生命单位，所有细胞肯定不一样。因此，从单细胞生成转录表达或基因组 DNA 图谱的技术至关重要。自 2009 年成立以来，单细胞 RNA 测序 (scRNA-seq) 已成为生物医学研究进展的主要推动力。在过去三年中，出现了几个新的单细胞测序平台。然而，对于这些常用平台的优势和局限性，只有少数系统比较。在这里，我们比较了两个单细胞测序平台：BD Rhapsody 和 10x Genomics Chromium，包括它们的不同机制以及用它们获得的一些 scRNA-seq 结果。