当前位置: X-MOL 学术Curr. Genomics › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Genome-wide identification, evolution and expression analysis of basic helix-loop-helix (bHLH) gene family in barley (Hordeum vulgare L.)
Current Genomics ( IF 1.8 ) Pub Date : 2020-12-21 , DOI: 10.2174/1389202921999201102165537
Qinglin Ke 1 , Wenjing Tao 1 , Tingting Li 1 , Wenqiu Pan 1 , Xiaoyun Chen 1 , Xiaoyu Wu 1 , Xiaojun Nie 1 , Licao Cui 1
Affiliation  

Background The basic helix-loop-helix (bHLH) transcription factor is one of the most important gene families in plants, playing a key role in diverse metabolic, physiological, and developmental processes. Although it has been well characterized in many plants, the significance of the bHLH family in barley is not well understood at present. Methods Through a genome-wide search against the updated barley reference genome, the genomic organization, evolution and expression of the bHLH family in barley were systematically analyzed. Results We identified 141 bHLHs in the barley genome (HvbHLHs) and further classified them into 24 subfamilies based on phylogenetic analysis. It was found that HvbHLHs in the same subfamily shared a similar conserved motif composition and exon-intron structures. Chromosome distribution and gene duplication analysis revealed that segmental duplication mainly contributed to the expansion of HvbHLHs and the duplicated genes were subjected to strong purifying selection. Furthermore, expression analysis revealed that HvbHLHs were widely expressed in different tissues and also involved in response to diverse abiotic stresses. The co-expression network was further analyzed to underpin the regulatory function of HvbHLHs. Finally, 25 genes were selected for qRT-PCR validation, the expression profiles of HvbHLHs showed diverse patterns, demonstrating their potential roles in relation to stress tolerance regulation. Conclusion This study reported the genome organization, evolutionary characteristics and expression profile of the bHLH family in barley, which not only provide the targets for further functional analysis, but also facilitate better understanding of the regulatory network bHLH genes involved in stress tolerance in barley.

中文翻译:

大麦(Hordeum vulgare L.)基本螺旋-环-螺旋(bHLH)基因家族的全基因组鉴定、进化和表达分析

背景 基本螺旋-环-螺旋(bHLH)转录因子是植物中最重要的基因家族之一,在多种代谢、生理和发育过程中发挥着关键作用。尽管它已在许多植物中得到很好的表征,但目前大麦中 bHLH 家族的重要性尚不清楚。方法通过对更新后的大麦参考基因组进行全基因组搜索,系统分析了大麦中bHLH家族的基因组组织、进化和表达。结果 我们在大麦基因组 (HvbHLHs) 中鉴定出 141 个 bHLH,并根据系统发育分析将它们进一步分为 24 个亚科。发现同一亚科中的 HvbHLHs 具有相似的保守基序组成和外显子-内含子结构。染色体分布和基因重复分析表明,片段重复主要促成了HvbHLHs的扩增,并且重复的基因受到了强烈的纯化选择。此外,表达分析表明,HvbHLHs 在不同组织中广泛表达,并且还参与了对各种非生物胁迫的反应。进一步分析了共表达网络以支持 HvbHLHs 的调节功能。最后,选择了 25 个基因进行 qRT-PCR 验证,HvbHLHs 的表达谱显示出不同的模式,证明了它们在应激耐受调节中的潜在作用。结论 本研究报道了大麦中 bHLH 家族的基因组结构、进化特征和表达谱,
更新日期:2020-12-21
down
wechat
bug