Proteoglycan (PG) is a type of glycoprotein which forms an extracellular matrix with collagen and hyaluronic acid to maintain articular cartilage, synovial membrane, and synovial fluid. This study aimed to evaluate the antiosteoarthritis effects of salmon nasal cartilage-derived PG in alleviating knee osteoarthritis in an osteoarthritis rat model. Knee osteoarthritis was induced in rats by intra-articular injection of monosodium iodoacetate (MIA), 3 mg/knee, to the right knee. Animals were then administered either diclofenac (3 mg/kg body weight [b.w]/day) or proteoglycan F (PGF; 40 mg/kg and 120 mg/kg b.w/day) by oral gavage for 6 consecutive weeks. Knee diameters were measured throughout the experimental period; serum interleukin-1β and tumor necrosis factor-alpha (TNF-α) levels, and histological analysis of the ligament were carried out at the end of the experiment. Salmon cartilage PG considerably alleviated the osteoarthritis symptoms in the model and lowered the serum concentrations of interleukin-1β and TNF-α. Diclofenac 3 mg/kg/day and PGF at doses of 40 mg/kg/day and 120 mg/kg/day also improved articular cartilage structure on further histological studies. This study demonstrated the in vivo effect of salmon cartilage PG in attenuating symptoms in an MIA-induced rat model, including reduction of inflammatory markers and histological improvement of cartilage tissue.

蛋白聚糖（PG）是一种糖蛋白，与胶原蛋白和透明质酸形成细胞外基质，以维持关节软骨，滑膜和滑液。这项研究旨在评估鲑鱼鼻软骨来源的PG在缓解骨关节炎大鼠模型中的膝关节骨关节炎中的抗骨关节炎作用。通过向右膝关节内注射3 mg /膝的碘乙酸单钠（MIA），在大鼠中诱发膝关节骨关节炎。然后，连续6周通过口服管饲法给动物施用双氯芬酸（3mg / kg体重[bw] /天）或蛋白聚糖F（PGF； 40mg / kg和120mg / kg bw /天）。在整个实验过程中测量膝盖的直径；血清白介素-1β和肿瘤坏死因子-α（TNF-α）的水平，在实验结束时进行韧带的组织学分析。鲑鱼软骨PG大大减轻了模型中的骨关节炎症状，并降低了白介素1β和TNF-α的血清浓度。在进一步的组织学研究中，双氯芬酸3 mg / kg /天和PGF 40 mg / kg /天和120 mg / kg /天的剂量也改善了关节软骨的结构。这项研究证明了鲑鱼软骨PG在减轻MIA诱导的大鼠模型中的症状方面的体内作用，包括减轻炎症标志物和改善软骨组织的组织学。在进一步的组织学研究中，双氯芬酸3 mg / kg /天和PGF 40 mg / kg /天和120 mg / kg /天的剂量也改善了关节软骨的结构。这项研究证明了鲑鱼软骨PG在减轻MIA诱导的大鼠模型中的症状方面的体内作用，包括减轻炎症标志物和改善软骨组织的组织学。在进一步的组织学研究中，双氯芬酸3 mg / kg /天和PGF 40 mg / kg /天和120 mg / kg /天的剂量也改善了关节软骨的结构。这项研究证明了鲑鱼软骨PG在减轻MIA诱导的大鼠模型中的症状方面的体内作用，包括减轻炎症标志物和改善软骨组织的组织学。