In the wild, being able to recognize and remember specific locations related to food sources and the associated attributes of landmarks is a cognitive trait important for survival. In the present work we show that the crab Neohelice granulata can be trained to associate a specific environment with an appetitive reward in a conditioned place preference task. After a single training trial, when the crabs were presented with a food pellet in the target quadrant of the training arena, they were able to form a long-term memory related to the event. This memory was evident at least 24 h after training and was protein-synthesis dependent. Importantly, the target area of the arena proved to be a non-neutral environment, given that animals initially avoided the target quadrant. In the present work we introduce for the first time an associative one-trial memory paradigm including a conditioned stimulus with a clear valence performed in a crustacean.

中文翻译：

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学习非中性条件刺激：在蟹新螺旋颗粒中放置偏好

在野外，能够识别和记住与食物来源有关的特定位置以及地标的相关属性是对生存至关重要的认知特征。在当前的工作中，我们表明可以训练螃蟹Neohelice granulata将特定的环境与有条件的地点偏爱任务中的奖励结合起来。经过一次培训后，当在培训场所的目标象限中向螃蟹提供食物颗粒时，它们就可以形成与事件有关的长期记忆。这种记忆在训练后至少24小时是明显的，并且是蛋白质合成依赖性的。重要的是，鉴于动物最初避开了目标象限，因此竞技场的目标区域被证明是非中性环境。