In the present study, we investigated cytogenetic and oxidative [total antioxidant capacity (TAC), total oxidant status (TOS)] effects of methanol and water extracts of Cladonia chlorophaea (Flörke ex Sommerf.) Sprengel, Dermatocarpon miniatum (L.) W.Mann and Parmelia saxatilis (L.) Ach. on cultured human lymphocytes. In addition, different phenolic compounds in the extracts were quantified by high performance liquid chromatography (HPLC) analysis. As a result of HPLC analysis, methanol extracts of all lichen species tested had higher phenolic compounds. Likewise, methanol extracts of each lichen increased TAC levels in lymphocytes more than water extracts. The TOS levels of the cells treated with different concentrations (1–100 mg/L) of the extracts decreased due to the increasing concentration of the extracts. Genotoxicity experiments revealed that the tested lichen extracts did not significantly increase ( p > 0.05) the level of genotoxicity on human peripheral lymphocyte culture compared to the negative control group. The results showed that C. chlorophaea , D. miniatum and P. saxatilis lichens, which were found to be a rich source of phenolic compounds, might be of interest in the pharmaceutical and food industries.

中文翻译：

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三种地衣提取物对人外周淋巴细胞的细胞遗传和氧化作用

在本研究中，我们研究了 Cladonia chlorophaea (Flörke ex Sommerf.) Sprengel、Dermatocarpon miniatum (L.) W. 甲醇和水提取物的细胞遗传学和氧化 [总抗氧化能力 (TAC)、总氧化状态 (TOS)] 的影响Mann 和 Parmelia saxatilis (L.) Ach。在培养的人类淋巴细胞上。此外，提取物中的不同酚类化合物通过高效液相色谱 (HPLC) 分析进行定量。HPLC 分析的结果是，所有测试地衣物种的甲醇提取物都含有较高的酚类化合物。同样，每种地衣的甲醇提取物比水提取物更能增加淋巴细胞中的 TAC 水平。由于提取物浓度的增加，用不同浓度 (1-100 mg/L) 提取物处理的细胞的 TOS 水平降低。基因毒性实验表明，与阴性对照组相比，受试地衣提取物对人外周淋巴细胞培养物的基因毒性水平没有显着增加（p > 0.05）。结果表明，被发现富含酚类化合物的 C. chlorophaea、D. miniatum 和 P. saxatilis 地衣可能在制药和食品工业中受到关注。