Abstract

This current study mainly focuses on synthesis of silver nanoparticles in an eco-friendly method using Cissus quadrangularis (CqNp), its characterization and understanding its potential biomedical applications. The CqNp’s were confirmed using UV-vis spectroscopy, FT-IR and the structure of the nanoparticles were analyzed by FESEM and EDAX. Anti-oxidant assay conducted with the plant and the CqNp’s, against the standard ascorbic acid showed its potential to be an antioxidant agent. α-amylase inhibition assay revealed the effectiveness of the CqNp’s which were almost similar to that of the standard acarbose. Molecular docking was performed for the plant compounds kaempferol and quercetin to find its binding energy, interacting residues and type of interaction. To the best of our knowledge, this is the first report on confirming the antioxidant and α-amylase inhibition potential of Cissus quadrangularis mediated nanoparticles (CqNps). Hence, this green approach can be further utilized to develop a potent anti-diabetic drug.

摘要

目前的研究主要集中在使用Cissus quadrangularis 以环保方法合成银纳米粒子（CqNp），其表征和了解其潜在的生物医学应用。使用紫外-可见光谱、FT-IR 确认了 CqNp，并通过 FESEM 和 EDAX 分析了纳米颗粒的结构。用植物和 CqNp 对标准抗坏血酸进行的抗氧化试验表明它具有作为抗氧化剂的潜力。α-淀粉酶抑制试验表明 CqNp 的有效性几乎与标准阿卡波糖相似。对植物化合物山奈酚和槲皮素进行分子对接，以发现其结合能、相互作用的残基和相互作用的类型。据我们所知，这是第一份关于确认Cissus quadrangularis的抗氧化和 α-淀粉酶抑制潜力的报告介导的纳米粒子（CqNps）。因此，这种绿色方法可以进一步用于开发有效的抗糖尿病药物。