ABSTRACT

This study aimed to compare the antifungal, antioxidant and cytotoxic activities of Aspergillus terreus mediated silver nanoparticles (AgNPs) with that of gold nanoparticles (AuNPs). AgNPs were spherical in shape with a size of 8–20 nm; whereas, AuNPs showed anisotropic morphology (10–50 nm). Interestingly, AgNPs showed excellent antifungal activity against all the plant pathogens tested; while, AuNPs did not show any inhibition. However, both these nanoparticles showed good antioxidant activities. Among six different cancer cell lines tested, AgNPs showed comparatively significant cytotoxicity against MCF-7 cells with an IC₅₀ value of 2.90 μg/mL; whereas, AuNPs showed the maximum activity against HepG2 cells (IC₅₀: 25.79 μg/mL). Acridine orange-ethidium bromide (AO-EB) dual staining and nucleic acid staining (DAPI) confirmed the apoptotic phenomenon induced by nanoparticles, which was further confirmed by cell cycle analysis. Taken together, myco-derived AgNPs can be considered for potential biomedical and agricultural applications after thoroughly analysing their toxicity.

摘要

本研究旨在比较土曲霉介导的银纳米粒子 (AgNPs) 与金纳米粒子 (AuNPs) 的抗真菌、抗氧化和细胞毒活性。AgNPs 呈球形，大小为 8-20 nm；而金纳米粒子表现出各向异性形态（10-50 nm）。有趣的是，AgNPs 对所有测试的植物病原体都表现出优异的抗真菌活性。而AuNPs没有表现出任何抑制作用。然而，这两种纳米颗粒都显示出良好的抗氧化活性。在测试的六种不同癌细胞系中，AgNPs 对 MCF-7 细胞表现出相对显着的细胞毒性，IC ₅₀值为 2.90 μg/mL；而 AuNPs 对 HepG2 细胞的活性最大（IC ₅₀: 25.79 微克/毫升)。吖啶橙-溴化乙锭（AO-EB）双重染色和核酸染色（DAPI）证实了纳米粒子诱导的细胞凋亡现象，细胞周期分析进一步证实了这一点。总之，在彻底分析其毒性后，可以考虑将真菌衍生的 AgNPs 用于潜在的生物医学和农业应用。