Abstract

Agave marmorata Roezl is an endemic succulent specie from the Oaxaca-Puebla area of Mexico. This plant is a medicinal recourse and contain a rich variety of saponins-type compounds with multiples biological effects. Some of them have been shown to be anticancer, antibacterial, or having anti-inflammatory and immunoregulation effects. This paper is the first scientific report to describe the pharmacological activity and chemistry of the saponin smilagenin-3-O-[β-D-glucopyranosyl (1→2)-β-D-galactopyranoside] (1), isolated from Agave marmorata Roezl. Saponin (1) displayed immunomodulating activity when assayed on cultured macrophages. It inhibits NO production (EC₅₀ = 5.6 mg/ml, E_max = 101%), as well as NF-κB expression (EC₅₀ = 0.086 mg/ml, E_max = 90%). Using bioinformatic molecular docking, we identified a new smilagenin- PI3K kinase interaction site.

摘要

龙舌兰 marmorata Roezl是墨西哥瓦哈卡-普埃布拉地区特有的多肉植物。这种植物是一种药用植物，含有丰富的皂苷类化合物，具有多种生物效应。其中一些已被证明具有抗癌、抗菌或抗炎和免疫调节作用。本文是第一篇描述皂苷异菝葜皂苷元-3-O-[β-D-吡喃葡萄糖基(1→2)-β-D-吡喃半乳糖苷] ( 1 ) 的药理活性和化学性质的科学报告，该皂苷从龙舌兰marmorata Roezl中分离出来。. 在培养的巨噬细胞上测定时，皂苷 ( 1)显示出免疫调节活性。它抑制 NO 的产生（EC ₅₀ = 5.6 mg/ml，E _max= 101%)，以及 NF-κB 表达 (EC ₅₀ = 0.086 mg/ml, E _max = 90%)。使用生物信息学分子对接，我们确定了一个新的异菝葜皂苷元-PI3K 激酶相互作用位点。