Abstract

Two undescribed diarylheptanoids, 3-(R)-acetyl-1-(3′,4′-dihydroxyphenyl)-7-(4′′-hydroxy-3′′ -methoxyphenyl)-heptane (1) and 11-Hydroxy-1,17-epoxy-7-(2-hydroxylphenyl)-13-(16-methoxyphenyl)-heptane (2) together with known compounds, namely, 11-Oxo-1,17-epoxy-7-(2-hydroxylphenyl)-13-(16-methoxyphenyl)-heptane (3) 3,4,5-Trihydroxytetralone (4) 4,8- Dihydroxytetralone (5), 4,5-Dihydroxytetralone (6), 5,8-Dihydroxy-3-methoxytetralone (7) were isolated from ethyl acetate extract of the green husk of Carya illinoinensis. The structures of compounds were established on the basis of IR, ¹H NMR, ¹³C NMR, DEPT, HSQC, HMBC, COSY spectroscopic and ESI-MS analysis. The isolated compounds were evaluated for AChE (acetylcholinesterase inhibition) and observed that compound 5 was potent inhibitor with IC₅₀ of 101.48 ± 4.00 µg/mL.

摘要

两种未描述的二芳基庚烷，3-( R )-乙酰基-1-(3',4'-二羟基苯基)-7-(4''-羟基-3''-甲氧基苯基)-庚烷( 1 )和11-羟基-1 ,17-epoxy-7-(2-hydroxylphenyl)-13-(16-methoxyphenyl)-heptane ( 2 ) 与已知化合物，即 11-Oxo-1,17-epoxy-7-(2-hydroxylphenyl)- 13-(16-甲氧基苯基)-庚烷 ( 3 ) 3,4,5-三羟基四氢萘酮 ( 4 ) 4,8-二羟基四氢萘酮 ( 5 ), 4,5-二羟基四氢萘酮 ( 6 ), 5,8-二羟基-3-甲氧基四氢萘酮 ( 7 )从伊利诺山核桃绿壳的乙酸乙酯提取物中分离得到。化合物的结构是在 IR 的基础上建立的，¹H NMR、¹³ C NMR、DEPT、HSQC、HMBC、COZY 光谱和 ESI-MS 分析。对分离的化合物进行 AChE（乙酰胆碱酯酶抑制）评估，并观察到化合物5是有效的抑制剂，IC ₅₀为 101.48 ± 4.00 µg/mL。