Abstract

In this work, a polar stationary phase was prepared by thermal immobilization of poly(glycidoxypropylmethyl-co-dimethylsiloxane) onto silica particles to separate polar compounds using water-rich mobile phases. The retention properties of the stationary phase were characterized by the separation of Tanaka and NIST SRM^® 870 test mixtures. The elution order of the compounds indicated the reversed-phase mode, but the stationary phase was less hydrophobic and with higher silanophilic activity than typical stationary phases. Polar compounds presented reasonable retention in the stationary phase and symmetrical peaks in the chromatograms, due to hydrophilic interactions with glycidoxy groups in the polymer side chain. The stationary phase was applied for the separation of small polar and ionizable compounds. The retention factors increased with the aqueous mobile phase component. So, all polar compounds were separated efficiently and eluted symmetrically under highly aqueous mobile phases, >90%. Per aqueous liquid chromatography was the principal separation mode of the new stationary phase, presenting as a green alternative to the hydrophilic interaction liquid chromatography mode for the separation of polar compounds.

摘要

在这项工作中，通过将聚（环氧丙氧基丙基甲基-co-二甲基硅氧烷）热固定在二氧化硅颗粒上以使用富水流动相分离极性化合物来制备极性固定相。固定相的保持性能进行了表征由Tanaka和NIST SRM的分离^®870种测试混合物。化合物的洗脱顺序表明是反相模式，但固定相的疏水性比典型固定相低，并且具有更高的嗜硅活性。极性化合物由于与聚合物侧链中的环氧丙氧基基团发生亲水性相互作用，因此在固定相中具有合理的保留度，在色谱图中具有对称峰。固定相用于分离小的极性化合物和可电离的化合物。保留因子随水性流动相组分的增加而增加。因此，所有极性化合物均能有效分离，并在> 90％的高水相流动相中对称洗脱。每水性液相色谱法是新固定相的主要分离方式，