The Australian redclaw crayfish Cherax quadricarinatus is a freshwater crustacean aquacultured in many countries. Redclaw crayfish are often exposed to saline waters in attempts to improve flavor, to relieve stress during transport, and to prevent or treat parasitic infestations. However, the effect of salinity on the crayfish osmoregulatory process is not well studied. In the present work, we assessed the effect of various salinities on survival, growth, and osmoregulation in redclaw crayfish. Adult crayfish were maintained at seven salinities (0, 2, 4, 6, 8, 10, and 12 ppt) for 4 weeks, and hemolymph osmolality, gill sodium potassium ATPase activity, and sodium potassium chloride cotransporter expression were assessed. In another experiment, juvenile crayfish were size sorted to similar weights and stocked at four salinities (0, 2, 4, and 6 ppt) for 8 weeks. The animals were group weighed every 2 weeks and individually weighed at the end of the eighth week. Hemolymph osmolality was constant as salinity increased from 0 to 10 ppt then a significant increase was observed at 12 ppt. Redclaw growth rate decreased with an increase in salinity from 0 to 6 ppt. Sodium potassium ATPase activity and sodium potassium chloride cotransporter expression increased with salinity increase. Although salinity might help treat ectoparasites, improve taste, and reduce transportation stress, long‐term exposure increases osmoregulatory metabolic costs and affects growth and energy consumption.

中文翻译：

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盐度对红爪小龙虾Cherax quadricarinatus存活，生长，血淋巴重量摩尔渗透压浓度，g钠ATP酶活性和氯化钠钾共转运蛋白表达的影响

澳大利亚红爪小龙虾Cherax quadricarinatus是在许多国家/地区养殖的淡水甲壳动物。红爪小龙虾经常暴露于盐水中，以试图改善风味，减轻运输过程中的压力以及预防或治疗寄生虫侵扰。然而，盐度对小龙虾渗透调节过程的影响尚未得到很好的研究。在目前的工作中，我们评估了各种盐度对小爪小龙虾的存活，生长和渗透调节的影响。将成年小龙虾维持在七个盐度（0、2、4、6、8、10和12个百分点）下4周，并评估了血淋巴渗透压、,钠ATP酶活性和氯化钠钾共转运蛋白的表达。在另一项实验中，将小龙虾的大小分类至相似的重量，并以四种盐​​度（0、2、4和6个百分点）放养8周。每2周对动物分组称重，并在第八周结束时分别称重。当盐度从0增加到10 ppt时，血淋巴重量摩尔渗透压浓度恒定，然后在12 ppt处观察到显着增加。随着盐度的增加，红爪的生长速率从0下降到6个百分点。盐度增加，钠钾ATP酶活性和氯化钠钾共转运蛋白表达增加。尽管盐度可能有助于治疗体外寄生虫，改善口感并减轻运输压力，但长期接触会增加渗透调节性代谢成本并影响生长和能量消耗。随着盐度的增加，红爪的生长速率从0下降到6个百分点。盐度增加，钠钾ATP酶活性和氯化钠钾共转运蛋白表达增加。尽管盐度可能有助于治疗体外寄生虫，改善口感并减轻运输压力，但长期接触会增加渗透调节性代谢成本并影响生长和能量消耗。随着盐度的增加，红爪的生长速率从0下降到6个百分点。盐度增加，钠钾ATP酶活性和氯化钠钾共转运蛋白表达增加。尽管盐度可能有助于治疗体外寄生虫，改善口感并减轻运输压力，但长期接触会增加渗透调节性代谢成本并影响生长和能量消耗。