Biocatalytic reduction of biomass-derived 5-hydroxymethylfurfural (HMF) to 2,5-bis(hydroxymethyl)furan (BHMF) is considered as a sustainable method and has attracted great attention. In this work, a yeast-like fungus, Aureobasidium subglaciale F134 was discovered as a whole-cell biocatalyst for BHMF synthesis from selective reduction of HMF. Its resting cells exhibited high tolerance and excellent selectivity to HMF in a broad pH range. The catalytic capacity of the cells can be substantially improved by Zn²⁺ ion. Under the optimized reaction conditions, 180 mM HMF substrate was converted into BHMF with a yield of 82 % and 97 % selectivity in 9 h. In a fed-batch conversion, 430 mM of BHMF product was obtained within 15 h, giving a productivity of 3.7 g/l per hour. In addition, it was found that the cells could also selectively convert furfural to form furfuryl alcohol. Discovery of A. subglaciale F134 cells for BHMF synthesis broadens the biocatalyitc toolbox for sustainable production of bio-based chemicals and biofuels from biomass-derived HMF. In addition, the results provide more information regarding the identification of related enzymes in future work.

将生物质衍生的5-羟甲基糠醛（HMF）生物催化还原为2,5-双（羟甲基）呋喃（BHMF）是一种可持续的方法，并引起了广泛关注。在这项工作中，发现了一种酵母样真菌，Aureobasidium subglaciale F134作为通过选择性还原HMF合成BHMF的全细胞生物催化剂。它的静止细胞在宽的pH范围内表现出高耐受性和对HMF的优异选择性。Zn ²⁺可以大大提高细胞的催化能力离子。在优化的反应条件下，将180 mM HMF底物在​​9小时内转化为BHMF，产率为82％，选择性为97％。在分批补料转化中，在15小时内获得了430 mM BHMF产品，生产率为每小时3.7 g / l。另外，发现细胞还可以选择性地将糠醛转化为糠醇。的发现A. subglaciale为BHMF合成F134细胞拓宽了由生物质衍生的HMF可持续生产的生物基化学品和生物燃料的biocatalyitc工具箱。此外，结果为将来工作中有关酶的鉴定提供了更多信息。