Abstract

Ginseng (Panax ginseng C. A. Meyer) is a famous Traditional Chinese Medicine, which is widely used to treat cardiovascular disease. Monascus ruber (M. ruber) is a fungus used in food and medicine fermentation, and lovastatin, its metabolite, is used extensively in the treatment of dyslipidemia. In this study, ginseng has been fermented by M. ruber, and the response surface methodology (RSM) was applied to optimize fermentation parameters to obtain optimal fermentation system, with further exploring to lipid-lowering activity of P. ginseng C. A. Meyer–M. ruber fermentation products (PM). The concentration of ginseng, temperature, and rotating speed were set as variables and the lovastatin yield was optimized by a Box–Behnken design (BBD) analyzed by RSM. The binding capacity of PM for sodium taurocholate and sodium cholate was assayed by UV spectrophotometry. The highest content of lovastatin production (85.53 μg g⁻¹) was obtained at a ginseng concentration of 1.96%, temperature of 30.11 °C, and a rotating speed of 160.47 rpm. PM exhibited bile acid binding capacity, which was stronger than unfermented ginseng. The RSM can be used to optimize the fermentation system to obtain the best fermentation process. In addition, the fermentation of ginseng by M. ruber can enhance the lipid-lowering effect.

抽象的

人参（Panax ginseng CA Meyer）是著名的中药，被广泛用于治疗心血管疾病。红曲霉（M.曲霉）是食品和药品的发酵，和洛伐他汀，其代谢物使用的真菌，在治疗血脂异常的广泛使用。在这项研究中，人参已经通过发酵M.曲霉，和响应面分析法（RSM）施加到优化发酵参数以获得最佳的发酵系统，进一步探索到的降脂活性P.人参CA迈耶- M.橡皮发酵产品（PM）。人参浓度，温度和旋转速度设置为变量，洛伐他汀产量通过RSM分析的Box-Behnken设计（BBD）进行优化。通过紫外分光光度法测定PM对牛磺胆酸钠和胆酸钠的结合能力。在人参浓度为1.96％，温度为30.11℃，转速为160.47 rpm的情况下，洛伐他汀的产量最高（85.53μgg ^-1）。PM的胆汁酸结合能力强于未发酵的人参。RSM可用于优化发酵系统以获得最佳发酵过程。另外，通过红掌鼠发酵人参可以增强降脂作用。