Polyunsaturated fatty acids (PUFAs) are essential in healthy diets and their production is extremely important. Natural sources of PUFAs includes animal and aquatic products such as marine fish oil, however there are several limitations such as the decrease of fish stocks throughout the world. Thus, microbial oils are a preferable source of PUFAs. Herein, it was studied the production of PUFAs by Mortierella alpina under solid-state fermentation (SSF) using polyurethane foam as inert substrate and synthetic medium or lignocellulosic hydrolysate as source of C, N, and other nutrients. Several parameters of fermentation conditions were evaluated as carbon source, inductors addition, ratio C/N and temperature. The highest amount of total PUFAs per mass of solid (535.41 ± 24.12 mg/g), linoleic acid (129.66 ± 5.84 mg/g), and α-linoleic acid (401.93 ± 18.10 mg/g) were produced when the culture medium contained 20 g/L glucose, 10% (w/v) linseed oil, the C/N ratio was adjusted to 25 and the incubation temperature was 25°C for 3 days decreasing to 16°C on the remaining 4 days of fermentation. In addition, a hemicellulosic hydrolysate can be used as low-cost substrate to produce PUFAs, although the production was lower than the achieved with synthetic medium. SSF showed an interesting technology for microbial PUFAs production.

中文翻译：

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高山被孢霉在聚氨酯泡沫上固态发酵生产多不饱和脂肪酸

多不饱和脂肪酸 (PUFA) 在健康饮食中必不可少，它们的生产极为重要。多不饱和脂肪酸的天然来源包括动物和水产品，例如海洋鱼油，但存在一些限制，例如全世界鱼类资源的减少。因此，微生物油是 PUFA 的优选来源。本文研究了高山被孢霉生产PUFAs在固态发酵 (SSF) 下，使用聚氨酯泡沫作为惰性基质，合成培养基或木质纤维素水解物作为 C、N 和其他营养物质的来源。发酵条件的几个参数被评估为碳源、诱导剂添加、C/N比和温度。当培养基含有20 g/L 葡萄糖，10% (w/v) 亚麻籽油，C/N 比调整为 25，孵育温度为 25°C，持续 3 天，发酵剩余 4 天降至 16°C。此外，半纤维素水解物可用作生产 PUFA 的低成本底物，尽管产量低于使用合成培养基实现的产量。