This study aimed to evaluate the probiotic additive on tilapia growth by establishing growth curves, as well as assessing the morphology of the muscular and ventral subcutaneous adipose tissues, carcass characteristics and gene expression that controls growth. Fingerlings of 5 g were grown in a recirculating aquaculture system consisting of 15 water tanks (0.25 m³) at 80 fish/m³. Fish were fed three times daily with the same feed, using additive with Bacillus subtilis, Bifidobacterium bifidum, Enterococcus faecium, Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus lactis, Lactobacillus plantarum and Pediococcus acidilactici (1.0 × 10⁹ CFU/g). They were assessed levels of 0.1% and 0.2% of additive probiotic homogenized in 2% soy oil and sprinkled over the feed. Exponential models were fit to compare growth rates, weighing samples at days 0, 30, 60 and 90 of cultivation. The probiotic mixture used in this study as feed additive may be diffused in aquaculture in order to increase productivity, as demonstrated by feed conversion rate, final weight and growth (13%–37% for 0.1% and 0.2% of additive level respectively). Growth stimulation occurred by white muscle fibre hypertrophy, accompanied by inhibition of MyoD and myostatin, gene expression and increase in the number of intestinal villi, ensuring greater absorption and utilization of nutrients provided in the diet.

中文翻译：

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益生菌添加剂影响尼罗罗非鱼（Oreochromis niloticus）的肌肉生长

这项研究旨在通过建立生长曲线来评估益生菌添加剂对罗非鱼的生长，以及评估肌肉和腹侧皮下脂肪组织的形态，car体特征和控制生长的基因表达。在由15个水箱（0.25 m ³）组成的循环水产养殖系统中以80鱼/米^3的密度养殖5克鱼种。鱼每天三次分别饲喂相同的进料，使用具有添加剂枯草芽孢杆菌，两歧双歧杆菌，肠球菌，嗜酸乳杆菌，干酪乳杆菌，乳杆菌乳酸乳球菌，植物乳杆菌和乳酸双球菌（1.0×10 ⁹ CFU / g）。他们评估了在2％的豆油中均质化并撒在饲料上的0.1％和0.2％的添加益生菌的水平。拟合指数模型以比较生长速度，在培养的第0、30、60和90天称重样品。本研究中用作饲料添加剂的益生菌混合物可以在水产养殖中进行扩散，以提高生产率，如饲料转化率，最终重量和生长（分别为添加剂含量的0.1％和0.2％的13％–37％）所证明的。白肌纤维肥大会刺激生长，并伴有MyoD和myostatin抑制，基因表达和肠绒毛数量增加，从而确保饮食中营养的吸收和利用。