Neurovespina is a synthetic peptide modified from Occidentalin-1202, a nine amino acid residue peptide isolated from the venom of the social wasp Polybia occidentalis. Previous studies showed that this peptide has a neuroprotective effect on the central nervous system, but its action on the eye has not been explored. So, the objective of this work was to investigate the neuroprotective effect of Neurovespina on the retina and its angiogenic potential in the chicken chorioallantoic membrane (CAM). Retinal ischemia was induced in rats by acute elevation of intraocular pressure (IOP). Electroretinography (ERG) measurements, histopathological and immunohistochemical analysis, and transmission electronic microscopy (TEM) records were performed to check the neuroprotection effect of Neurovespina in the retina of the animals. The angiogenic activity of the peptide was investigated by CAM assay. The results showed that Neurovespina was able to reduce the effects induced by ischemic injury, preventing the reduction of a- and b-waves in the scotopic ERG. Histopathological and immunohistochemistry assays showed that Neurovespina, mainly at 60 μg/ml, protected all layers of the retina. The CAM assay revealed that the peptide promoted the reduction of CAM vessels. So, Neurovespina was able to protect retinal cells from ischemic insult and has an antiangiogenic effect, which can be considered as a promising neuroprotective agent for intravitreal application.

中文翻译：

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Neurovespina 可减轻缺血引起的视网膜损伤，Neurovespina 是一种来自西黄蜂毒液的肽


Neurovespina 是一种由 Occidentalin-1202 修饰的合成肽，Occidentalin-1202 是从社会黄蜂 Polybia occidentalis 的毒液中分离出来的九个氨基酸残基肽。此前的研究表明，这种肽对中枢神经系统具有神经保护作用，但其对眼睛的作用尚未被探索。因此，这项工作的目的是研究 Neurovespina 对视网膜的神经保护作用及其在鸡绒毛尿囊膜 (CAM) 中的血管生成潜力。眼内压（IOP）急性升高可诱发大鼠视网膜缺血。通过视网膜电图 (ERG) 测量、组织病理学和免疫组织化学分析以及透射电子显微镜 (TEM) 记录来检查 Neurovespina 对动物视网膜的神经保护作用。通过CAM测定研究肽的血管生成活性。结果表明，Neurovespina 能够减少缺血性损伤引起的影响，防止暗视 ERG 中 a 波和 b 波的减少。组织病理学和免疫组织化学检测表明，Neurovespina（主要浓度为 60 μg/ml）可以保护视网膜的所有层。 CAM 测定显示该肽促进 CAM 血管的减少。因此，Neurovespina 能够保护视网膜细胞免受缺血性损伤，并具有抗血管生成作用，可以被认为是玻璃体内应用的一种有前景的神经保护剂。