Abstract

Investigation of original drugs involves a study of pharmacokinetics and metabolism. Most investigations use HPLC/MS analysis. With the use of the active ingredient of a non-benzodiazepine anxiolytic Fabomotizole [2-(2-morpholinoethylthio)-5-ethoxybenzylimidazole dihydrochloride)] and its metabolite as an example, it was found that capillary gas chromatography–mass spectrometry with positive ion chemical ionization can be applied to detect insignificant amounts of polar biologically active substances, which are chemically stable during the passage of the gas chromatographic system; however, the analytes can be lost at different stages of the analysis.

中文翻译：

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气相色谱/质谱联用正离子化学电离法检测抗焦虑型Fabomozole及其代谢产物的活性物质

摘要

原始药物的研究涉及药代动力学和代谢研究。大多数调查使用HPLC / MS分析。以非苯二氮杂类抗焦虑型依法替莫唑[2-（2-吗啉代乙硫基）-5-乙氧基苄基咪唑二盐酸盐]的活性成分及其代谢物为例，发现毛细管气相色谱-质谱联用正离子化学电离可用于检测极少量的极性生物活性物质，这些物质在气相色谱系统通过期间化学稳定；但是，分析物可能会在分析的不同阶段丢失。