Abstract

Eleutherococcus senticosus is a plant with medicinal properties due to the presence of glycosides, called eleutherosides А, В and В₁, C, D, E, and F, in its roots and rhizomes. In this paper, a procedure for the simultaneous determination of Eleutheroside B and Eleutheroside E in dry samples of Eleutherococcus senticosus roots from different manufactures by high performance liquid chromatography/tandem mass spectrometry was proposed. The quantification of eleutheroside B and eleutheroside E was performed using electrospray ionization in the negative ion mode with detection by multiple reaction monitoring (MRM), which ensured a better signal-to-noise ratio. To isolate eleutherosides from plant materials, simple and rapid methods of sample preparation were used, including extraction in an ultrasonic bath with various solvents. The calibration curve was linear in the concentration range from 40 to 4000 ng/mL.

中文翻译：

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液相色谱/质谱法测定刺肠大肠球菌提取物中的大黄苷B和大黄苷E

摘要

刺五加是具有医学性质的植物，由于糖苷的存在下，被称为刺五加А，В和В ₁，C，d，E，和F，在其根和根茎。本文同时测定了刺五加刺肠球菌干样品中的伊洛糖苷B和伊洛糖苷E提出了通过高效液相色谱/串联质谱分析来自不同制造商的根。使用负离子模式下的电喷雾电离，通过多反应监测（MRM）进行检测，从而可测定eleutheroside B和eleutheroside E的定量，从而确保了更好的信噪比。为了从植物材料中分离出苦菊苷，使用了简单而快速的样品制备方法，包括在超声波浴中用各种溶剂进行提取。校准曲线在40至4000 ng / mL的浓度范围内呈线性关系。