A feeding trial was conducted with inclusion levels of Cajanus cajan leafmeal (CCLM) in the diet of Labeo rohita to study the growth, haematological indices, digestive enzyme, physio‐metabolic changes and molecular expression of insulin‐like growth factor‐1 (IGF‐1), insulin‐like growth factor‐binding protein‐1 (IGFBP‐1), insulin‐like growth factor‐1 receptor (IGF‐1R) genes. Four practical diets with CCLM, control, T20 (20%), T30 (30%) and T40 (40%) were prepared. Weight gain%, SGR, FER and PER were significantly (p < 0.05) higher when 30% de‐oiled rice bran (DORB) was replaced by CCLM whereas the treatment group 40% registered with lower FCR value. The protease and amylase activities were higher in the T30 group. T20 showed significantly higher (p < 0.05) hepatic and muscular lactate dehydrogenase and serum glucose was also higher, whereas, WBC content was found significantly (p < 0.05) higher in control. The hepatic IGF‐1 gene expression was significantly higher (p < 0.05) in T40 followed by the T30 group but the weight gain was not significantly (p > 0.05) higher in T30 from the T40 group. Results reveal that the response of IGF‐1, IGF‐1R and IGFBP‐1 genes was complementary to the biological growth parameters and hence were used as an indicator to evaluating growth. The study also demonstrates that CCLM can replace 100% DORB in the diet of L. rohita without any antinutritional or adverse growth effects.

中文翻译：

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木豆（Cajanus cajan）叶粉的包被对Labeo rohita（Hamilton，1822）幼鱼生长，生理代谢和免疫参数以及IGF-1，IGF-1R和IGFBP-1基因表达的影响

在Labeo rohita的饮食中进行了一项含Cajanus cajan叶粉（CCLM）含量的喂养试验，以研究胰岛素样生长因子1（IGF-）的生长，血液学指标，消化酶，生理代谢变化和分子表达。 1），胰岛素样生长因子结合蛋白1（IGFBP-1），胰岛素样生长因子1受体（IGF-1R）基因。使用CCLM，对照，T20（20％），T30（30％）和T40（40％）配制了四种实用饮食。 当用CCLM代替30％的脱油米糠（DORB）时，体重增加％，SGR，FER和PER显着提高（p <0.05），而40％的治疗组的FCR值较低。T30组的蛋白酶和淀粉酶活性较高。T20表现出更高的p  <0.05）肝和肌肉乳酸脱氢酶和血清葡萄糖也更高，而对照组的WBC含量则明显 更高（p <0.05）。 T40组接着T30组的肝IGF-1基因表达明显较高（p <0.05），而T40组的T30组的体重增加并不显着（p  > 0.05）。结果显示，IGF-1，IGF-1R和IGFBP-1基因的反应与生物生长参数互补，因此被用作评估生长的指标。该研究还表明，章法可以替代的食物100％DORB L.野鲮没有任何抗营养或不利生长的影响。