Adulteration of aquafeed has raised critical concern regarding quality and safety of aquatic meats. This study assessed the impact of melamine (MEL) and melamine‐formaldehyde (MEF) chemicals (adulterants) on haematology, gill and liver antioxidant enzymes activities of Clarias gariepinus. Fish were fed diets contaminated with MEL and MEF at 3.0 g Kg⁻¹ (0.3%) dietary inclusion for 45 days, followed by a depuration trial that lasted for 30 days. Body biomass of MEL‐ and MEF‐treated groups decreased markedly, relative to the control. Haematological parameters such as red blood cell, white blood cell, haemoglobin and packed cell volume contents of the treated groups reduced prominently, while antioxidant enzymes (superoxide dismutase, catalase and glutathione peroxidase) activities were suppressed, particularly in the liver of the treated groups. Additionally, toxicity of MEL and MEF caused significant increase in malondialdehyde (MDA) content in the gill and liver tissues of the exposed groups. However, antioxidant enzymes activities and haematology were restored to the normal physiological condition, during the recovery (depuration) phase. MEL‐ and MEF‐induced toxic effects on the exposed fish and the findings of the current study provides baseline information and insight on the adverse impact of these chemicals in aquatic models.

中文翻译：

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水产饲料中掺有三聚氰胺和三聚氰胺-甲醛化学品的掺假；异位研究对Clarias gariepinus血液学和抗氧化系统的影响

水产饲料的掺假引起了对水生肉质量和安全的严重关注。这项研究评估了三聚氰胺（MEL）和三聚氰胺-甲醛（MEF）化学品（掺和剂）对非洲胡子Cl（Clarias gariepinus）的血液学，g和肝脏抗氧化酶活性的影响。用3.0 g Kg ^-1饲喂鱼类的食物，这些食物被MEL和MEF污染（0.3％）纳入饮食45天，然后进行了持续30天的净化试验。相对于对照组，MEL和MEF处理组的身体生物量显着下降。治疗组的血液学参数（如红细胞，白细胞，血红蛋白和堆积细胞体积含量）显着降低，而抗氧化酶（超氧化物歧化酶，过氧化氢酶和谷胱甘肽过氧化物酶）的活性受到抑制，尤其是在治疗组的肝脏中。此外，MEL和MEF的毒性导致暴露组the和肝组织中丙二醛（MDA）含量显着增加。但是，在恢复（净化）阶段，抗氧化酶的活性和血液学已恢复到正常的生理状态。