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Comparison of concentrated fresh mononuclear cells and cultured mesenchymal stem cells from bone marrow for bone regeneration
STEM CELLS Translational Medicine ( IF 5.4 ) Pub Date : 2020-12-19 , DOI: 10.1002/sctm.20-0234 Fengzhou Du 1, 2 , Qian Wang 1 , Long Ouyang 1 , Huanhuan Wu 1 , Zhigang Yang 1 , Xin Fu 1 , Xia Liu 1 , Li Yan 1 , Yilin Cao 1 , Ran Xiao 1
STEM CELLS Translational Medicine ( IF 5.4 ) Pub Date : 2020-12-19 , DOI: 10.1002/sctm.20-0234 Fengzhou Du 1, 2 , Qian Wang 1 , Long Ouyang 1 , Huanhuan Wu 1 , Zhigang Yang 1 , Xin Fu 1 , Xia Liu 1 , Li Yan 1 , Yilin Cao 1 , Ran Xiao 1
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Autologous bone marrow mononuclear cell (BMMNC) transplantation has been widely studied in recent years. The fresh cell cocktail in BMMNCs, without going through the in vitro culture process, helps to establish a stable microenvironment for osteogenesis, and each cell type may play a unique role in bone regeneration. Our study compared the efficacy of concentrated fresh BMMNCs and cultured bone marrow‐derived mesenchymal stem cells (BMSCs) in Beagle dogs for the first time. Fifteen‐millimeter segmental bone defects were created in the animals' tibia bones. In BMMNCs group, the defects were repaired with concentrated fresh BMMNCs combined with β‐TCP (n = 5); in cultured BMSC group, with in vitro cultured and osteo‐induced BMSCs combined with β‐TCP (n = 5); in scaffold‐only group, with a β‐TCP graft alone (n = 5); and in blank group, nothing was grafted (n = 3). The healing process was monitored by X‐rays and single photon emission computed tomography. The animals were sacrificed 12 months after surgery and their tibias were harvested and analyzed by microcomputed tomography and hard tissue histology. Moreover, the microstructure, chemical components, and microbiomechanical properties of the regenerated bone tissue were explored by multiphoton microscopy, Raman spectroscopy and nanoindentation. The results showed that BMMNCs group promoted much more bone regeneration than cultured BMSC group. The grafts in BMMNCs group were better mineralized, and they had collagen arrangement and microbiomechanical properties similar to the contralateral native tibia bone. These results indicate that concentrated fresh bone marrow mononuclear cells may be superior to in vitro expanded stem cells in segmental bone defect repair.
中文翻译：
浓缩新鲜单核细胞和骨髓间充质干细胞用于骨再生的比较
近年来，自体骨髓单个核细胞（BMMNC）移植得到了广泛的研究。BMMNCs中的新鲜细胞混合物，无需经过体外培养过程，有助于建立稳定的成骨微环境，每种细胞类型都可能在骨再生中发挥独特的作用。我们的研究首次比较了浓缩的新鲜 BMMNCs 和培养的骨髓间充质干细胞 (BMSCs) 在 Beagle 犬中的疗效。在动物的胫骨中产生了 15 毫米的节段性骨缺损。BMMNCs组采用浓缩新鲜BMMNCs联合β-TCP修复缺陷（n = 5）；在培养的 BMSC 组中，体外培养和骨诱导的 BMSCs 联合 β-TCP (n = 5)；在仅支架组中，仅使用 β-TCP 移植物（n = 5）；在空白组中，没有嫁接（n = 3）。通过 X 射线和单光子发射计算机断层扫描监测愈合过程。手术后 12 个月处死动物，采集胫骨并通过微计算机断层扫描和硬组织组织学进行分析。此外，通过多光子显微镜、拉曼光谱和纳米压痕研究了再生骨组织的微观结构、化学成分和微生物力学特性。结果表明，BMMNCs 组比培养的 BMSC 组促进了更多的骨再生。BMMNCs组移植物矿化较好，胶原排列和微生物力学特性与对侧原生胫骨相似。
更新日期：2020-12-19
中文翻译：
浓缩新鲜单核细胞和骨髓间充质干细胞用于骨再生的比较
近年来，自体骨髓单个核细胞（BMMNC）移植得到了广泛的研究。BMMNCs中的新鲜细胞混合物，无需经过体外培养过程，有助于建立稳定的成骨微环境，每种细胞类型都可能在骨再生中发挥独特的作用。我们的研究首次比较了浓缩的新鲜 BMMNCs 和培养的骨髓间充质干细胞 (BMSCs) 在 Beagle 犬中的疗效。在动物的胫骨中产生了 15 毫米的节段性骨缺损。BMMNCs组采用浓缩新鲜BMMNCs联合β-TCP修复缺陷（n = 5）；在培养的 BMSC 组中，体外培养和骨诱导的 BMSCs 联合 β-TCP (n = 5)；在仅支架组中，仅使用 β-TCP 移植物（n = 5）；在空白组中，没有嫁接（n = 3）。通过 X 射线和单光子发射计算机断层扫描监测愈合过程。手术后 12 个月处死动物，采集胫骨并通过微计算机断层扫描和硬组织组织学进行分析。此外，通过多光子显微镜、拉曼光谱和纳米压痕研究了再生骨组织的微观结构、化学成分和微生物力学特性。结果表明，BMMNCs 组比培养的 BMSC 组促进了更多的骨再生。BMMNCs组移植物矿化较好，胶原排列和微生物力学特性与对侧原生胫骨相似。
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