The inability to thoroughly examine diatom type material existing only in resin‐mounted permanent slides is a common frustration for diatomologists. Here, we present an efficient, inexpensive, and straightforward technique to extract siliceous exoskeletons of diatoms from permanent slides prepared with commonly used mounting media. Van Heurck’s slide constituting an isotype duplicate of Proschkinia bulnheimii var. belgica, an entity with an uncertain taxonomic status, was deconstructed to allow thorough examination using scanning electron microscopy. Numerous specimens of the taxon, including intact frustules and specimens with complete areola occlusions, were recovered and documented. The extracted diatom material was sufficient to prepare two scanning electron microscopy specimens and reconstruct the permanent slide. The proposed approach may help overcome many of the taxonomic bottlenecks associated with the low resolution of information provided by older diatom descriptions and permanent slide observations.

中文翻译：

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永久性载玻片中的硅藻型材料不需要永久不用于电子显微镜检查

对于硅藻学家而言，无法彻底检查仅存在于树脂安装的永久性载玻片中的硅藻类材料是一个普遍的问题。在这里，我们提出了一种高效，廉价且直接的技术，可从使用常用安装介质制备的永久性载玻片中提取硅藻的硅质外骨骼。Van Heurck的幻灯片构成了Proschkinia bulnheimii  var的同型复制 。 比利时，具有不确定分类状态的实体，被解构以允许使用扫描电子显微镜进行彻底检查。收集并记录了许多分类单元标本，包括完整的壳和完整的乳晕闭塞标本。提取的硅藻材料足以制备两个扫描电子显微镜标本并重建永久性玻片。所提出的方法可能有助于克服许多与较旧的硅藻描述和永久性滑坡观测所提供的信息的低分辨率相关的分类瓶颈。