The textile dyeing and printing industries has led to extensive environmental pollution and severely threatens ecosystems. The best microbial species for such application was selected among the isolated bacterial populations by conducting CI Reactive Blue 40 (CI RB 40) batch degradation studies with the bacterial-algal-probiotic strains. In this study, three suitable species (Pseudomonas putida, Chlorella and Lactobacillus plantarum) were applied to degrade and detoxify CI RB 40, a reactive diazo dye in Real Textile Wastewater, used in textile dyeing industry worldwide. Process parameters were optimized using Response Surface Methodology and under the optimum conditions (e.g., inoculum size of 10%), temperature of 35 °C, 150 ppm, and time of 6 days). The maximum COD and color removal efficiencies, when tested with 1000 ppm of dye using batch reactors were found to be 89% and 99%, respectively. Our results showed also that bacteria had a high decolorization capacity. The regression analysis revealed a good match of the experimental data to the second-order polynomial with a high coefficient of determination (R²). UV–Visible and FTIR spectroscopy analysis confirmed the biodegradation of CI RB 40. Finally, toxicity of CIRB 40 before and after biodegradation was studied and the detoxification of CIRB 40 dye solution after biodegradation process was confirmed.

纺织印染业造成了广泛的环境污染，严重威胁着生态系统。通过对细菌-藻类-益生菌菌株进行 CI Reactive Blue 40 (CI RB 40) 批量降解研究，从分离的细菌种群中选择了最适合此类应用的微生物物种。在本研究中，三种适宜的菌种（恶臭假单胞菌、小球藻和植物乳杆菌）) 用于降解和解毒 CI RB 40，这是一种真实纺织废水中的活性重氮染料，用于全球纺织染色行业。使用响应面方法并在最佳条件（例如，10% 的接种量）、35°C 的温度、150 ppm 和 6 天的时间下优化工艺参数。当使用间歇反应器以 1000 ppm 染料进行测试时，发现最大 COD 和颜色去除效率分别为 89% 和 99%。我们的结果还表明，细菌具有很高的脱色能力。回归分析显示实验数据与具有高决定系数 (R ²）。紫外-可见光和 FTIR 光谱分析证实了 CI RB 40 的生物降解性。最后，研究了生物降解前后 CIRB 40 的毒性，并确认了生物降解过程后 CIRB 40 染料溶液的解毒作用。