With an increase in the obese population, the indiscriminate demand for anti-obesity drugs for rapid weight loss or maintenance has grown. As a result, illegal substances that could induce unexpected negative health effects or fatal side effects are being produced and mixed into consumer products. In the present study, the metabolites of five major illegal anti-obesity drugs are analyzed for the first time. Our data can be utilized to identify related compounds and predict their toxicological effects. Didesmethylsibutramine, desmethylsibutramine, homosibutramine, chlorosibutramine, and benzylsibutramine were metabolized in in vitro and in vivo models, and the metabolites were identified using liquid chromatography quadrupole-time of flight mass spectrometry (LC-Q-TOF-MS) and tandem mass spectrometry (LC-Q-TOF-MS/MS). The in vivo metabolite analysis was carried out using urine and feces samples from rats, and the in vitro metabolite analysis was performed by incubating the analogues with human liver microsomes. We found that each sibutramine analogue was metabolized into several constituents: 2 (M1–2), 5 (M1–5), 11 (M1–11), 7 (N1–7), and 5 (O1–5). In conclusion, our metabolic study could be used for toxicological detection of illegal obesity treatments and metabolite identification in forensic cases.

随着肥胖人口的增加，对用于快速减肥或维持体重的抗肥胖药的需求不断增长。结果，正在产生可能引起意想不到的负面健康影响或致命副作用的非法物质，并将其混入消费品中。在本研究中，首次对五种主要的抗肥胖药物的代谢产物进行了分析。我们的数据可用于识别相关化合物并预测其毒理作用。在体外和体内代谢了二甲基西布曲明，去甲基西布曲明，高西布曲明，氯西布曲明和苄基西布曲明用液相色谱四极杆飞行时间质谱（LC-Q-TOF-MS）和串联质谱（LC-Q-TOF-MS / MS）鉴定代谢产物。的体内代谢物分析是使用尿和粪便样品从大鼠中，以及在体外代谢物分析是通过将与人肝微粒的类似物执行的。我们发现每个西布曲明类似物都代谢成几种成分：2（M1-2），5（M1-5），11（M1-1），7（N1-7）和5（O1-5）。总之，我们的代谢研究可用于法医案例中非法肥胖治疗的毒理学检测和代谢物鉴定。