Herein, we present an alternative approach to obtain a highly sensitive and stable self-powered biosensor that was used to detect D-fructose as proof of concept.In this platform, we perform a two-step process, viz. self-charging the biosupercapacitor for a constant time by using D-fructose as fuel and using the stored charge to realize the detection of D-fructose by performing several polarization curves at different D-fructose concentrations. The proposed BSC shows an instantaneous power density release of 17.6 mW cm⁻² and 3.8 mW cm⁻² in pulse mode and at constant load, respectively. Moreover, the power density achieved for the self-charging BSC in pulse mode or under constant load allows for an enhancement of the sensitivity of the device up to 10 times (3.82 ± 0.01 mW cm⁻² mM⁻¹, charging time = 70 min) compared to the BSC in continuous operation mode and 100 times compared to the normal enzymatic fuel cell. The platform can potentially be employed as a self-powered biosensor in food or biomedical applications.

在本文中，我们提出了一种替代方法来获得高度敏感且稳定的自供电生物传感器，该传感器用于检测D-果糖作为概念验证。在此平台上，我们执行两步过程，即。通过使用D-果糖作为燃料，对生物超级电容器进行恒定时间的自动充电，并使用存储的电荷通过在不同的D-果糖浓度下执行几条极化曲线来实现对D-果糖的检测。拟议的BSC显示瞬时功率密度释放为17.6 mW cm ^-2和3.8 mW cm ^-2分别在脉冲模式和恒定负载下。此外，在脉冲模式或恒定负载下为自充电BSC实现的功率密度可将设备的灵敏度提高多达10倍（3.82±0.01 mW cm ^-2 mM ^-1，充电时间= 70分钟）与BSC（在连续操作模式下）相比，是正常酶燃料电池的100倍。该平台有可能在食品或生物医学应用中用作自供电生物传感器。