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Dynamic approach for mono- and di-fermentation of black liquor and livestock wastewater for 2-bio-(H2&CH4) production
Biomass & Bioenergy ( IF 5.8 ) Pub Date : 2020-12-18 , DOI: 10.1016/j.biombioe.2020.105947
Ahmed Tawfik , Gamal K. Hassan , Zhong Yu , Hala A. Salah , Marwa Hassan , Fangang Meng

Mono-and di-fermentation of black liquor (BL) and livestock wastewater (LW) was extensively investigated in batch assay experiments. Methane production was the dominant in mono-fermentation of BL and partially co-digested with LW (90% BL+10% LW). Increasing the fraction of LW in the feedstock shifted the microbial activities from biomethanisation into bio-hydrogen generation process. The hydrogen potential (A) was significantly increased from 468 ± 12 to 969 ± 10 ml at increasing the LW ratio in the di-fermentation process from 30% LW + 70% BL to 50% LW + 50% BL. The enzymes activities of α-amylase, xylanase, CM-cellulase, polygalacturonase and protease were maximized at a levels of 420 ± 2.5, 509 ± 5.4, 560 ± 3.9, 686 ± 7.9 and 127.2 ± 6.3 U/100 ml respectively for di-fermentation of 50% LW + 50% BL. Similarly, the valerate (HVa), butyrate (HBu) and acetate (HAc) was highly increased in the digestate of 50% LW + 50% BL up to 654 ± 122.9, 2464.6 ± 67.9 and 657 ± 11.8 mg/l respectively. The Methanobacterium and Methanosaeta were dominated for 10% LW + 90% BL and hydrogen producers i.e., Clostridium, was the major for di-fermentation of the batches containing 50% LW + 50% BL.



中文翻译:

黑液和牲畜废水单发酵和发酵的2-bio-(H 2&CH 4)生产的动态方法

在批处理实验中,对黑液(BL)和牲畜废水(LW)的单发酵和双发酵进行了广泛研究。甲烷的生产在BL的单发酵中占主导地位,并与LW(90%BL + 10%LW)一起被部分消化。原料中LW含量的增加使微生物活性从生物甲烷化转变为生物氢生成过程。在将发酵过程中的LW比从30%LW + 70%BL增加到50%LW + 50%BL时,氢势(A)从468±12 ml显着增加到969±10 ml。二淀粉酶的α-淀粉酶,木聚糖酶,CM-纤维素酶,聚半乳糖醛酸酶和蛋白酶的酶活性分别达到420±2.5、509±5.4、560±3.9、686±7.9和127.2±6.3 U / 100 ml的水平50%LW + 50%BL的发酵。同样,戊酸酯(HVa)在50%LW + 50%BL的消化物中,丁酸(HBu)和乙酸盐(HAc)分别显着增加,分别达到654±122.9、2464.6±67.9和657±11.8 mg / l。的甲烷杆菌甲烷菌以10%LW + 90%BL为主,制氢剂,即梭状芽胞杆菌是发酵含50%LW + 50%BL的批次的主要原料。

更新日期:2020-12-20
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