Mono-and di-fermentation of black liquor (BL) and livestock wastewater (LW) was extensively investigated in batch assay experiments. Methane production was the dominant in mono-fermentation of BL and partially co-digested with LW (90% BL+10% LW). Increasing the fraction of LW in the feedstock shifted the microbial activities from biomethanisation into bio-hydrogen generation process. The hydrogen potential (A) was significantly increased from 468 ± 12 to 969 ± 10 ml at increasing the LW ratio in the di-fermentation process from 30% LW + 70% BL to 50% LW + 50% BL. The enzymes activities of α-amylase, xylanase, CM-cellulase, polygalacturonase and protease were maximized at a levels of 420 ± 2.5, 509 ± 5.4, 560 ± 3.9, 686 ± 7.9 and 127.2 ± 6.3 U/100 ml respectively for di-fermentation of 50% LW + 50% BL. Similarly, the valerate (HVa), butyrate (HBu) and acetate (HAc) was highly increased in the digestate of 50% LW + 50% BL up to 654 ± 122.9, 2464.6 ± 67.9 and 657 ± 11.8 mg/l respectively. The Methanobacterium and Methanosaeta were dominated for 10% LW + 90% BL and hydrogen producers i.e., Clostridium, was the major for di-fermentation of the batches containing 50% LW + 50% BL.

在批处理实验中，对黑液（BL）和牲畜废水（LW）的单发酵和双发酵进行了广泛研究。甲烷的生产在BL的单发酵中占主导地位，并与LW（90％BL + 10％LW）一起被部分消化。原料中LW含量的增加使微生物活性从生物甲烷化转变为生物氢生成过程。在将发酵过程中的LW比从30％LW + 70％BL增加到50％LW + 50％BL时，氢势（A）从468±12 ml显着增加到969±10 ml。二淀粉酶的α-淀粉酶，木聚糖酶，CM-纤维素酶，聚半乳糖醛酸酶和蛋白酶的酶活性分别达到420±2.5、509±5.4、560±3.9、686±7.9和127.2±6.3 U / 100 ml的水平50％LW + 50％BL的发酵。同样，戊酸酯（HVa）在50％LW + 50％BL的消化物中，丁酸（HBu）和乙酸盐（HAc）分别显着增加，分别达到654±122.9、2464.6±67.9和657±11.8 mg / l。的甲烷杆菌和甲烷菌以10％LW + 90％BL为主，制氢剂，即梭状芽胞杆菌是发酵含50％LW + 50％BL的批次的主要原料。