TRIzol is used for the extraction of RNA, DNA and proteins from tissues or cells. Here, we present a simple picking method to extract DNA from tissues using TRIzol. Spectrophotometric analysis showed that the 260/280 and 260/230 nm optical density ratio of the picking method's DNA is ideal and better than that obtained by the classic TRIzol method. Gel electrophoresis showed that there was no RNA contamination, and the DNA had not degraded. DNA extracted by the picking method had the same performance in restriction enzyme digestion and quantitative PCR as that obtained by the traditional method. Viral DNA in the infected tissue was also obtained. This modified method facilitates various molecular biology assays.

中文翻译：

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使用基于 TRIzol 的方法从动物组织中提取高纯度 DNA。

TRIzol 用于从组织或细胞中提取 RNA、DNA 和蛋白质。在这里，我们提出了一种使用 TRIzol 从组织中提取 DNA 的简单挑选方法。分光光度分析表明，采摘法DNA的260/280和260/230 nm光密度比是理想的，优于经典TRIzol法获得的光密度比。凝胶电泳显示没有RNA污染，DNA没有降解。挑取法提取的DNA在限制性内切酶消化和定量PCR方面与传统方法提取的DNA具有相同的性能。还获得了受感染组织中的病毒 DNA。这种改进的方法促进了各种分子生物学测定。