Aedes aegypti is the primary vector of arthropod-borne viruses and one of the most widespread and invasive mosquito species. Due to the lack of efficient specific drugs or vaccination strategies, vector population control methods, such as the sterile insect technique, are receiving renewed interest. However, availability of a reliable genetic sexing strategy is crucial, since there is almost zero tolerance for accidentally released females. Development of genetic sexing strains through classical genetics is hindered by genetic recombination that is not suppressed in males as is the case in many Diptera. Isolation of naturally-occurring or irradiation-induced inversions can enhance the genetic stability of genetic sexing strains developed through genetically linking desirable phenotypes with the male determining region. For the induction and isolation of inversions through irradiation, 200 male pupae of the ‘BRA’ wild type strain were irradiated at 30 Gy and 100 isomale lines were set up by crossing with homozygous ‘red-eye’ (re) mutant females. Recombination between re and the M locus and the white (w) gene (causing a recessive white eye phenotype when mutated) and the M locus was tested in 45 and 32 lines, respectively. One inversion (Inv35) reduced recombination between both re and the M locus, and wand the M locus, consistent with the presence of a rather extended inversion between the two morphological mutations, that includes the M locus. Another inversion (Inv5) reduced recombination only between w and the M locus. In search of naturally-occurring, recombination-suppressing inversions, homozygous females from the red eye and the white eye strains were crossed with seventeen and fourteen wild type strains collected worldwide, representing either recently colonized or long-established laboratory populations. Despite evidence of varying frequencies of recombination, no combination led to the elimination or substantial reduction of recombination. Inducing inversions through irradiation is a feasible strategy to isolate recombination suppressors either on the M or the m chromosome for Aedes aegypti. Such inversions can be incorporated in genetic sexing strains developed through classical genetics to enhance their genetic stability and support SIT or other approaches that aim to population suppression through male-delivered sterility.

中文翻译：

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辐照诱导的反转抑制埃及伊蚊 M 基因座和形态标记之间的重组


埃及伊蚊是节肢动物传播的病毒的主要载体，也是分布最广、最具入侵性的蚊种之一。由于缺乏有效的特异性药物或疫苗接种策略，媒介种群控制方法，例如昆虫不育技术，重新受到人们的关注。然而，可靠的基因性别鉴定策略的可用性至关重要，因为对意外释放的雌性几乎零容忍。通过经典遗传学发展遗传性别鉴定品系受到遗传重组的阻碍，而遗传重组在雄性中并未像许多双翅目中那样受到抑制。分离自然发生的或辐射诱导的倒位可以增强通过将所需表型与雄性决定区域遗传连接而开发的遗传性别鉴定菌株的遗传稳定性。为了通过辐射诱导和分离倒转，对 200 个“BRA”野生型品系的雄性蛹进行 30 Gy 的辐射，并通过与纯合“红眼”（re）突变雌性杂交建立 100 个异麦芽品系。 re 和 M 基因座以及白色 (w) 基因（突变时导致隐性白眼表型）和 M 基因座之间的重组分别在 45 和 32 个品系中进行了测试。一种倒位 (Inv35) 减少了 re 和 M 基因座之间的重组，并减少了 M 基因座之间的重组，这与两个形态突变（包括 M 基因座）之间存在相当广泛的倒位一致。另一种倒位 (Inv5) 仅减少 w 和 M 基因座之间的重组。 为了寻找自然发生的、抑制重组的倒位，来自红眼和白眼品系的纯合雌性与世界各地收集的十七和十四个野生型品系杂交，这些野生型品系代表最近殖民或长期建立的实验室群体。尽管有证据表明重组频率不同，但没有任何组合能够消除或大幅减少重组。通过照射诱导倒位是分离埃及伊蚊 M 或 m 染色体上的重组抑制子的可行策略。这种倒位可以纳入通过经典遗传学开发的遗传性别鉴定品系中，以增强其遗传稳定性并支持昆虫不育技术或其他旨在通过雄性不育来抑制种群的方法。