Bactrocera dorsalis is a destructive polyphagous and highly invasive insect pest of tropical and subtropical species of fruit and vegetable crops. The sterile insect technique (SIT) has been used for decades to control insect pests of agricultural, veterinary, and human health importance. Irradiation of pupae in SIT can reduce the ecological fitness of the sterile insects. Our previous study has shown that a gut bacterial strain BD177 that could restore ecological fitness by promoting host food intake and metabolic activities. Using long-read sequence technologies, we assembled the complete genome of K. michiganensis BD177 strain. The complete genome of K. michiganensis BD177 comprises one circular chromosome and four plasmids with a GC content of 55.03%. The pan-genome analysis was performed on 119 genomes (strain BD177 genome and 118 out of 128 published Klebsiella sp. genomes since ten were discarded). The pan-genome includes a total of 49305 gene clusters, a small number of 858 core genes, and a high number of accessory (10566) genes. Pan-genome and average nucleotide identity (ANI) analysis showed that BD177 is more similar to the type strain K. michiganensis DSM2544, while away from the type strain K. oxytoca ATCC13182. Comparative genome analysis with 21 K. oxytoca and 12 K. michiganensis strains, identified 213 unique genes, several of them related to amino acid metabolism, metabolism of cofactors and vitamins, and xenobiotics biodegradation and metabolism in BD177 genome. Phylogenomics analysis reclassified strain BD177 as a member of the species K. michiganensis. Comparative genome analysis suggested that K. michiganensis BD177 has the strain-specific ability to provide three essential amino acids (phenylalanine, tryptophan and methionine) and two vitamins B (folate and riboflavin) to B. dorsalis. The clear classification status of BD177 strain and identification of unique genetic characteristics may contribute to expanding our understanding of the symbiotic relationship of gut microbiota and B. dorsalis.

中文翻译：

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密歇根氏克雷伯菌BD177及其相关成员的比较基因组学。揭示与背实蝇的共生关系

Bactrocera dorsalis是一种破坏性的多食性和高度入侵性的水果和蔬菜作物热带和亚热带物种的害虫。无菌昆虫技术（SIT）已被用于控制具有农业，兽医和人类健康重要性的害虫。在昆虫不育技术中辐照of可降低不育昆虫的生态适应性。我们以前的研究表明，肠道细菌BD177可通过促进宿主食物的摄入和代谢活动来恢复生态适应性。使用长读序列技术，我们组装了密歇根州立大学BD177菌株的完整基因组。密歇根州立大学BD177的完整基因组包含一个环形染色体和四个质粒，GC含量为55.03％。泛基因组分析是在119个基因组上进行的（菌株BD177基因组和128个已公布的克雷伯菌属基因组中的118个，因为十个被丢弃了）。泛基因组总共包括49305个基因簇，少数858个核心基因和大量辅助（10566）基因。泛基因组和平均核苷酸同一性（ANI）分析表明，BD177与密歇根州立菌株DSM2544更相似，而远离产氧耶尔森氏菌ATCC13182。用21 K. oxytoca和12 K. michiganensis菌株进行比较基因组分析，确定了213个独特基因，其中一些与氨基酸代谢，辅因子和维生素的代谢以及BD177基因组中异种生物的生物降解和代谢有关。系统生物学分析将BD177菌株重新归类为密歇根州种（K. michiganensis）。比较的基因组分析表明，密歇根州立大学BD177具有菌株特异性的能力，可为背叶芥菜提供三种必需氨基酸（苯丙氨酸，色氨酸和蛋氨酸）和两种维生素B（叶酸和核黄素）。BD177菌株的明确分类状态和独特遗传特征的鉴定可能有助于扩大我们对肠道菌群与背侧双歧杆菌共生关系的理解。