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A turn-on fluorescent probe for Lu3+ recognition and bio-imaging in live cells and zebrafish
Analytical Methods ( IF 2.7 ) Pub Date : 2020-11-26 , DOI: 10.1039/d0ay02060e Mujthaba Aatif A 1, 2, 3, 4, 5 , Selva Kumar R 1, 2, 3, 4, 5 , S. Abdul Majeed 5, 6, 7 , S. K. Ashok Kumar 1, 2, 3, 4, 5
Analytical Methods ( IF 2.7 ) Pub Date : 2020-11-26 , DOI: 10.1039/d0ay02060e Mujthaba Aatif A 1, 2, 3, 4, 5 , Selva Kumar R 1, 2, 3, 4, 5 , S. Abdul Majeed 5, 6, 7 , S. K. Ashok Kumar 1, 2, 3, 4, 5
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A new Lu3+ selective fluorescent probe L was synthesized and characterized. The optical properties of L were investigated by using absorption and fluorescence spectral studies in 7 : 3 (v/v) aqueous dimethyl sulphoxide. Upon addition of Lu3+ in a pH 4 (acetate buffer) solution of L, the weakly fluorescent probe L became highly fluorescent. The fluorescence intensity increased five-fold at 490 nm with excitation at 437 nm. The formation of 2 : 1 complexation between L and Lu3+ was confirmed by Job's plot. The binding constant (Ka, 1.43 × 104 M−1) was determined by the Benesi–Hildebrand (BH) method. The limit of detection (LOD) of Lu3+ using L was found to be 23 nM. The binding mechanism of L with Lu3+ was studied by 1H-NMR, ESI mass spectroscopy, and theoretical studies. Further, the probe L was successfully used to bioimage Lu3+ in a zebrafish gill cell line (DrG) and in the yolk, papillae of the eyes, and head of zebrafish embryos (Danio rerio), therefore providing a powerful live imaging approach for investigating chemical signaling in complex multicellular systems.
中文翻译：
用于在活细胞和斑马鱼中进行Lu3 +识别和生物成像的开启式荧光探针
合成并表征了新的Lu 3+选择性荧光探针L。通过在7：3（v / v）的二甲基亚砜水溶液中进行吸收和荧光光谱研究，研究了L的光学性质。在L的pH 4（乙酸缓冲液）溶液中添加Lu 3+时，弱荧光探针L变为高荧光。在490 nm激发下，荧光强度在490 nm处增加了五倍。乔布氏图证实了L和Lu 3+之间2：1络合物的形成。结合常数（K a，1.43×10 4M -1）由Benesi–Hildebrand（BH）方法确定。发现使用L对Lu 3+的检测极限（LOD）为23 nM。通过1 H-NMR，ESI质谱和理论研究研究了L与Lu 3+的结合机理。此外，探针L已成功用于在斑马鱼g细胞系（DrG）以及蛋黄，眼睛的乳头和斑马鱼胚胎的头部（Danio rerio）中对Lu 3+进行生物成像，从而提供了强大的实时成像方法研究复杂多细胞系统中的化学信号传导。
更新日期：2020-12-18
中文翻译：
用于在活细胞和斑马鱼中进行Lu3 +识别和生物成像的开启式荧光探针
合成并表征了新的Lu 3+选择性荧光探针L。通过在7：3（v / v）的二甲基亚砜水溶液中进行吸收和荧光光谱研究，研究了L的光学性质。在L的pH 4（乙酸缓冲液）溶液中添加Lu 3+时，弱荧光探针L变为高荧光。在490 nm激发下，荧光强度在490 nm处增加了五倍。乔布氏图证实了L和Lu 3+之间2：1络合物的形成。结合常数（K a，1.43×10 4M -1）由Benesi–Hildebrand（BH）方法确定。发现使用L对Lu 3+的检测极限（LOD）为23 nM。通过1 H-NMR，ESI质谱和理论研究研究了L与Lu 3+的结合机理。此外，探针L已成功用于在斑马鱼g细胞系（DrG）以及蛋黄，眼睛的乳头和斑马鱼胚胎的头部（Danio rerio）中对Lu 3+进行生物成像，从而提供了强大的实时成像方法研究复杂多细胞系统中的化学信号传导。
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