In recent decades, three dimensional (3D) bio-printing technology has found widespread use in tissue engineering applications. The aim of this study is to scrutinize different parameters of the bioprinter – with the help of simulation software – to print a hydrogel so much so that avoid high amounts of shear stress which is detrimental for cell viability and cell proliferation. Rheology analysis was done on several hydrogels composed of different percentages of components: alginate, collagen, and gelatin. The results have led to the combination of percentages collagen:alginate:gelatin (1:4:8)% as the best condition which makes sol-gel transition at room temperature possible. The results have shown the highest diffusion rate and cell viability for the cross-linked sample with 1.5% CaCl₂ for the duration of 1 h. Finally, we have succeeded in printing the hydrogel that is mechanically strong with suitable degradation rate and cell viability.

近几十年来，三维 (3D) 生物打印技术在组织工程应用中得到了广泛的应用。这项研究的目的是在模拟软件的帮助下仔细检查生物打印机的不同参数来打印水凝胶，以避免大量剪切应力，这对细胞活力和细胞增殖是有害的。对由不同百分比的组分组成的几种水凝胶进行流变学分析：藻酸盐、胶原蛋白和明胶。结果表明，胶原蛋白：藻酸盐：明胶 (1:4:8)% 的组合是使室温下溶胶-凝胶转变成为可能的最佳条件。结果表明，含 1.5% CaCl ₂的交联样品具有最高的扩散速率和细胞活力持续 1 小时。最后，我们成功地打印出机械强度高、降解率和细胞活力合适的水凝胶。