Novel the bilayered electrospun biosheet with rapid cell mimiciking and proliferative efficacy will be suitable for wound healing application. The optimized concentration of gelatin (G) and sodium alginate (A) biosheet with nanofibrous Poly (3-hydroxybutyric acid) (P) as a bilayered elctrospun matrix through electrospinning. The engineered GAP bilayered biosheet involves tissue formation at extra cellular matrix (ECM) which further characterized its function in vitro and invivo. Here we fabricated GAP which exhibit better physiochemical properties, biological and mechanical properties with superior prosomes it enhance air passable at skin wounds. The Bilayered biosheet matrix possess better biocompatibility, cell adherence, fructuous and cell to cell interactions evaluated using cell lines. Furthermore, GAP bilayered matrix regulates growth factors to attain maximum wound closure efficiency during invivo. Thus, the fabricated GAP electrospun biosheet would be a possible wound dressing for skin wound applications.

具有快速细胞模仿和增殖功效的新型双层电纺生物片将适合伤口愈合应用。通过静电纺丝将纳米纤维聚（3-羟基丁酸）（P）作为双层电纺丝基质的明胶（G）和藻酸钠（A）生物片的最佳浓度。经过改造的GAP双层生物片涉及在细胞外基质（ECM）处的组织形成，从而进一步表征了其在体外的功能和体内。在这里，我们制造的GAP表现出更好的理化特性，生物学和机械特性以及优异的蛋白质组，可以增强皮肤伤口处的空气流通性。双层生物片基质具有更好的生物相容性，细胞粘附性，果肉状和使用细胞系评估的细胞间相互作用。此外，GAP双层基质可调节生长因子，以在体内产生最大的伤口闭合效率。因此，制造的GAP电纺生物片将是用于皮肤伤口应用的可能的伤口敷料。