Environmental friendly and effective bio-augmentation is a very promising technology in the aniline wastewater treatment field. In this study, an aniline degradation bacterial strain was isolated from activated sludge, designated AD4. It was proved to be capable of removing aniline effectively in the concentration range between 200 and 800 mg/L within 72 h and could tolerate 1,400 mg/L of aniline, which was identified as Delftia tsuruhatensis. The influence of environmental factors containing initial aniline concentration, initial pH, temperature, shaker speed, salinity, and inoculum amount on the growth and degradation of strain AD4 were investigated. The results showed that AD4 had a wide range of suitable pH (5–8) and higher efficiency in a weak acidic environment than in weak base. The aniline concentration, pH, and temperature were used as independent parameters to optimize the aniline degradation by strain AD4, and a statistically significant (R² = 0.9817, p < 0.001) quadratic polynomial mathematical model was built. Meanwhile, a simulated reactor was designed to test the aniline-degradation ability of strain AD4 during the aeration stage. The degradation rate in 600 mg/L of aniline solution reached 51.5% after 8 h.

中文翻译：

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高效且抗苯胺降解菌株 AD4 的鉴定和表征

环保有效的生物强化是苯胺废水处理领域非常有前景的技术。在这项研究中，从活性污泥中分离出一种苯胺降解细菌菌株，命名为 AD4。经证明能在72 h内有效去除200-800 mg/L浓度范围内的苯胺，并能耐受1,400 mg/L的苯胺，鉴定为Delftia tsuruhatensis. 考察了初始苯胺浓度、初始pH、温度、摇床速度、盐度、接种量等环境因素对AD4菌株生长和降解的影响。结果表明，AD4 在弱酸性环境中具有较宽的适宜 pH（5-8）范围和比弱碱中更高的效率。苯胺浓度、pH 值和温度被用作独立参数来优化菌株 AD4 对苯胺的降解，并且具有统计学意义（R ²  = 0.9817，p < 0.001) 建立了二次多项式数学模型。同时，设计了模拟反应器来测试菌株AD4在曝气阶段的苯胺降解能力。在600mg/L苯胺溶液中8h降解率达到51.5%。