The establishment of human-induced pluripotent stem cell (iPSC) models from sporadic Alzheimer’s disease (sAD) patients is necessary and could potentially benefit research into disease etiology and therapeutic strategies. However, the development of sAD iPSC models is still limited due to the multifactorial nature of the disease. Here, we extracted peripheral blood mononuclear cells (PBMCs) from a patient with sAD and induced them into iPSC by introducing the Sendai virus expressing Oct3/4, Sox2, c-Myc, and Klf4, which were subsequently induced into neural cells to build the cell model of AD. Using alkaline phosphatase staining, immunofluorescence staining, karyotype analysis, reverse transcription-polymerase chain reaction (RT-PCR), and teratoma formation in vitro, we demonstrated that the iPSC derived from PMBCs (PBMC-iPSC) had a normal karyotype and potential to differentiate into three embryonic layers. Immunofluorescence staining and quantitative real-time polymerase chain reaction (qPCR) suggested that PBMC-iPSCs were successfully differentiated into neural cells. Detection of beta-amyloid protein oligomer (AβO), beta-amyloid protein 1-40 (Aβ 1-40), and beta-amyloid protein 1-42 (Aβ 1-42) indicated that the AD cell model was satisfactorily constructed in vitro. In conclusion, this study has successfully generated an AD cell model with pathological features of beta-amyloid peptide deposition using PBMC from a patient with sAD.

中文翻译：

---

通过散发患者外周血分子细胞体外重建阿尔茨海默氏病细胞模型

建立来自偶发性阿尔茨海默病（sAD）患者的人诱导多能干细胞（iPSC）模型是必要的，并且可能有益于疾病病因学和治疗策略的研究。然而，由于该疾病的多因素性质，sAD iPSC模型的开发仍然受到限制。在这里，我们从患有sAD的患者中提取外周血单核细胞（PBMC），并通过引入表达Oct3 / 4，Sox2，c-Myc和Klf4的仙台病毒将其诱导到iPSC中，随后将其诱导为神经细胞以构建神经胶质细胞。 AD的细胞模型。使用碱性磷酸酶染色，免疫荧光染色，核型分析，逆转录聚合酶链反应（RT-PCR）和体外畸胎瘤形成，我们证明了源自PMBC的iPSC（PBMC-iPSC）具有正常的核型，并具有分化为三个胚层的潜力。免疫荧光染色和定量实时聚合酶链反应（qPCR）表明PBMC-iPSC已成功分化为神经细胞。β-淀粉样蛋白寡聚物（A的检测β O），β-淀粉样蛋白1-40（A β 1-40）和β-淀粉样蛋白1-42（A β 1-42）表明，AD细胞模型是令人满意的体外构建。总之，这项研究成功地使用来自sAD患者的PBMC建立了具有β-淀粉样肽沉积病理特征的AD细胞模型。