Abstract Jute (Corchorus capsularis L.) is an annual herb of the bast fiber plant and has great potentials in food and medicinal usages because of its various bioactivities. In this study, ultrafiltration coupled with high-performance liquid chromatography-mass spectrometry was established for screening xanthine oxidase inhibitors from the jute leaves extract. Under the optimum screening conditions, three inhibitors were successfully screened and identified as chlorogenic acid, echinacoside, and isorhamnetin-rutinoside with UV and MS data. The fluorescent quenching analysis showed that three inhibitors quenched the fluorescence intensities of enzyme with different binding capacities. For further exploring the bioactivity of three inhibitors, the protective effects on hydrogen peroxide-induced oxidative stress was investigated using human normal liver cell (LO2), human gastric mucosal epithelial cell (GES-1), and human umbilical vein endothelial cell (HUVEC). As a result, they exhibited protective effects on three injured cells in dose-dependent manners without cytotoxicity. To evaluate the difference among different jute species obtained in our laboratories, the amounts of three compounds in ten samples were assessed and analyzed. The results showed that it could be divided into three groups. The jute leaves showed nutrient and medical potentials and deserved further research on pharmaceutical and biochemical utilization in future.

中文翻译：

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黄麻叶黄嘌呤氧化酶抑制剂的筛选与分析及其对过氧化氢诱导的细胞氧化应激的保护作用

摘要 黄麻（Corchorus capsularis L.）是韧皮纤维植物的一年生草本植物，由于其多种生物活性，在食用和药用方面具有很大的潜力。在这项研究中，建立了超滤结合高效液相色谱-质谱法从黄麻叶提取物中筛选黄嘌呤氧化酶抑制剂的方法。在最佳筛选条件下，成功筛选出绿原酸、松果菊苷和异鼠李素-芸香苷三种抑制剂，并利用UV和MS数据进行鉴定。荧光猝灭分析表明，三种抑制剂猝灭了不同结合能力的酶的荧光强度。为了进一步探索三种抑制剂的生物活性，使用人正常肝细胞 (LO2)、人胃粘膜上皮细胞 (GES-1) 和人脐静脉内皮细胞 (HUVEC) 研究了对过氧化氢诱导的氧化应激的保护作用。结果，它们以剂量依赖性方式对三种受损细胞表现出保护作用，而没有细胞毒性。为了评估在我们实验室获得的不同黄麻种类之间的差异，我们对十个样品中三种化合物的含量进行了评估和分析。结果表明，它可以分为三组。黄麻叶显示出营养和医疗潜力，值得在未来进一步研究药物和生化利用。它们以剂量依赖性方式对三种受损细胞表现出保护作用，而没有细胞毒性。为了评估在我们实验室获得的不同黄麻种类之间的差异，我们对十个样品中三种化合物的含量进行了评估和分析。结果表明，它可以分为三组。黄麻叶显示出营养和医疗潜力，值得在未来进一步研究药物和生化利用。它们以剂量依赖性方式对三种受损细胞表现出保护作用，而没有细胞毒性。为了评估在我们实验室获得的不同黄麻种类之间的差异，我们对十个样品中三种化合物的含量进行了评估和分析。结果表明，它可以分为三组。黄麻叶显示出营养和医疗潜力，值得在未来进一步研究药物和生化利用。