Force Spectroscopy Imaging and Constriction Assays Reveal the Effects of Graphene Oxide on the Mechanical Properties of Alginate Microcapsules,ACS Biomaterials Science & Engineering

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Force Spectroscopy Imaging and Constriction Assays Reveal the Effects of Graphene Oxide on the Mechanical Properties of Alginate Microcapsules
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2020-12-18 , DOI: 10.1021/acsbiomaterials.0c01382
María Virumbrales-Muñoz ₁ , Laura Paz-Artigas _{2,

3} , Jesús Ciriza _{4,

5} , Clara Alcaine _{2,

3,

5} , Albert Espona-Noguera _{4,

5} , Manuel Doblaré _{2,

3,

5} , Laura Sáenz del Burgo _{4,

5} , Kaoutar Ziani _{4,

5} , Jose Luis Pedraz _{4,

5} , Luis Fernández _{2,

3,

5} , Ignacio Ochoa _{2,

3,

5}

Affiliation

Microencapsulation of cells in hydrogel-based porous matrices is an approach that has demonstrated great success in regenerative cell therapy. These microcapsules work by concealing the exogenous cells and materials in a robust biomaterial that prevents their recognition by the immune system. A vast number of formulations and additives are continuously being tested to optimize cell viability and mechanical properties of the hydrogel. Determining the effects of new microcapsule additives is a lengthy process that usually requires extensive in vitro and in vivo testing. In this paper, we developed a workflow using nanoindentation (i.e., indentation with a nanoprobe in an atomic force microscope) and a custom-built microfluidic constriction device to characterize the effect of graphene oxide (GO) on three microcapsule formulations. With our workflow, we determined that GO modifies the microcapsule stiffness and surface properties in a formulation-dependent manner. Our results also suggest, for the first time, that GO alters the conformation of the microcapsule hydrogel and its interaction with subsequent coatings. Overall, our workflow can infer the effects of new additives on microcapsule surfaces. Thus, our workflow can contribute to diminishing the time required for the validation of new microcapsule formulations and accelerate their clinical translation.

中文翻译：

力谱成像和压缩分析揭示了氧化石墨烯对藻酸盐微胶囊力学性能的影响

在基于水凝胶的多孔基质中将细胞微囊化是一种在再生细胞治疗中取得巨大成功的方法。这些微胶囊通过将外源细胞和材料隐藏在坚固的生物材料中而起作用，从而阻止了它们被免疫系统识别。大量配方和添加剂正在不断测试中，以优化水凝胶的细胞活力和机械性能。确定新的微胶囊添加剂的作用是一个漫长的过程，通常需要大量的体外和体内试验测试。在本文中，我们开发了使用纳米压痕（即在原子力显微镜中用纳米探针压痕）和定制的微流体压缩装置的工作流程，以表征氧化石墨烯（GO）对三种微胶囊制剂的影响。通过我们的工作流程，我们确定GO以配方依赖的方式修饰了微胶囊的刚度和表面性质。我们的结果也首次表明，GO改变了微胶囊水凝胶的构象及其与后续涂层的相互作用。总体而言，我们的工作流程可以推断出新添加剂对微胶囊表面的影响。因此，我们的工作流程可有助于减少验证新微胶囊制剂所需的时间，并加快其临床翻译速度。

更新日期：2021-01-11

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