Abstract

Due to pollution in the process of chemical treatments, wool textile industry has been remarkably changed to use eco-friendly reagents in preparatory and finishing operations. We previously isolated a wool-degrading bacterial strain (Pseudomonas sp.3096-4) from decomposed wool fabrics, which produces a new type of keratinase capable of degrading cuticle layers. In this study, using fluorescein isothiocyanate (FITC) labelling and scanning electron microscope, we found that the Pseudomonas-derived keratinase efficiently removed the cuticle layer by protein hydrolysis and the degradation proceeded from surface to inner components of wool fibres. A 48-h treatment with the keratinase effectively removed cuticle scales on the wool surface with interior structure largely intact. This treatment significantly reinforced the strength and tenacity of wool fibres, showing its potential as a promising method in the wool textile industry.

摘要

由于化学处理过程中的污染，羊毛纺织工业已发生显着变化，在制备和后整理操作中使用了环保试剂。我们以前从分解的羊毛织物中分离出了一种降解羊毛的细菌菌株（Pseudomonas sp.3096-4），该菌株会产生一种新型的可降解表皮层的角蛋白酶。在这项研究中，使用异硫氰酸荧光素（FITC）标记和扫描电子显微镜，我们发现假单胞菌衍生的角蛋白酶通过蛋白质水解有效地去除了表皮层，并且降解从羊毛纤维的表面到内部组分进行。用角蛋白酶处理48小时可有效去除羊毛表面上的表皮鳞屑，内部结构基本保持完整。这种处理显着增强了羊毛纤维的强度和韧性，显示出它在羊毛纺织工业中作为有前途的方法的潜力。