ABSTRACT

Soil samples collected from different fruit orchards of Karnataka were used for isolation of Streptomyces spp. and all the isolates were screened for their plant growth-promoting (PGP) traits including phytohormones production viz. indole-3-acetic acid (IAA) and gibberellic acid (GA₃), nutrient (phosphate and zinc) solubilisation, siderophore production, enzyme activities (cellulase, tannase and xylanase) and antagonistic potential against soil borne fungal pathogens. Based on PGP traits as well as compatibilities, the following three Streptomyces spp. ‘S. avermitillis PAN Act2 (NAIMCC-B-01412), S. cinnamonesis PAN Act3 (NAIMCC-B-01413) and S. canus PAN Act5 (NAIMCC-B-01342)’ were selected for Streptomyces consortium (SMC) preparation using lignite as carrier material. Out of three isolates, S. canus registered significantly higher IAA and GA₃ production compared to other isolates. All three isolates had the potential to suppress the fungal phytopathogens viz. Cerotocystis fimbriata, Fusarium oxysporum, Macrophomina sp. and Sclerotium sp., with the levels of suppression ranging from 58.0% to 85.0%. The carrier-based SMC was subsequently evaluated in pomegranate to recover the plants from wilt like symptoms under field conditions. From this study, it was found that application of 50 gm of the SMC per plant through soil drenching at regular intervals (once every 2 months) had a significant impact on the recovery of the plant from wilt like symptoms after eight months. The present study is an addition to the growing knowledge on the utility of Streptomyces in fruit production.

摘要

从卡纳塔克邦不同果园收集的土壤样品用于分离链霉菌。和所有的分离筛选了植物生长促进（PGP）特性，包括植物激素生产即。吲哚-3-乙酸（IAA）和赤霉素（GA ₃），养分（磷酸盐和锌）增溶，铁载体生成，酶活性（纤维素酶，鞣酸酶和木聚糖酶）以及对土壤传播的真菌病原体的拮抗潜力。根据PGP的性状和相容性，以下三种链霉菌属（Streptomyces spp）。' S. avermitillis PAN ACT2（NAIMCC-B-01412），S. cinnamonesis PAN ACT3（NAIMCC-B-01413）和S. canusPAN Act5（NAIMCC-B-01342）'被选择用于使用褐煤作为载体材料的链霉菌联盟（SMC）的制备中。与其他分离株相比，在三种分离株中，加拿大链球菌的IAA和GA ₃产量显着提高。所有这三个株有抑制真菌植物病原体的潜在即。毛状绒头孢菌，尖孢镰刀菌，Macrophomina sp。和菌核sp。，抑制水平范围从58.0％到85.0％。随后在石榴中评估了基于载体的SMC，以在田间条件下从枯萎样症状中恢复植物。从这项研究中发现，定期（每2个月一次）通过土壤淋水每棵植物施用50克SMC，对八个月后植物从枯萎状症状的恢复具有重大影响。本研究是对链霉菌在水果生产中用途的不断增长的知识的补充。