The diatom Didymosphenia geminata can produce thick benthic mats along river bottoms, possibly in association with soluble reactive phosphorus mat-forming genetic variants, or gene expression associated with environmental variation. We isolated cells collected from mat-forming and non-mat-forming sites in British Columbia and Alberta, Canada and compared soluble reactive phosphorus, frequency of dividing cells and gene expression via RNA sequencing. Frequency of dividing cells and soluble reactive phosphorus were higher at the non-mat-forming site and there was no significant difference in frequency of dividing cells between Alberta and British Columbia sites, with one exception. Sixty-six gene products from reference diatom (Phaeodactylum tricornutum and Thalassiosira pseudonana) and bacterial (Caulobacter crescentus) genomes were expressed only in the mat-forming sample and many were related to phosphorus metabolism. One hundred seventy two genes were expressed only in the non-mat-forming sample and many were related to cell division and silica metabolism. Differential gene expression in the periphyton communities suggests that there might be different periphyton molecular phenotypes, including D. geminata, related to P acquisition and polysaccharide metabolism at the low P site and cell division and growth at the high P site.

中文翻译：

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Didymosphenia geminata 为主的附着生物垫茎形成、分裂细胞频率和基因表达的研究

硅藻 Didymosphenia geminata 可以沿着河底产生厚厚的底栖垫，这可能与可溶性活性磷垫形成遗传变异或与环境变化相关的基因表达有关。我们分离了从不列颠哥伦比亚省和加拿大阿尔伯塔省的垫形成和非垫形成位点收集的细胞，并通过 RNA 测序比较了可溶性活性磷、分裂细胞的频率和基因表达。非垫形成部位的分裂细胞频率和可溶性活性磷较高，阿尔伯塔省和不列颠哥伦比亚省的细胞分裂频率没有显着差异，只有一个例外。来自参考硅藻 (Phaeodactylum tricornutum 和 Thalassiosira pseudonana) 和细菌 (Caulobacter crescentus) 基因组的 66 种基因产物仅在形成垫的样品中表达，其中许多与磷代谢有关。172 个基因仅在非垫形成样品中表达，许多基因与细胞分裂和二氧化硅代谢有关。周生生物群落中的差异基因表达表明可能存在不同的周生生物分子表型，包括 D. geminata，与低磷位点的磷获取和多糖代谢以及高磷位点的细胞分裂和生长有关。172 个基因仅在非垫形成样品中表达，许多基因与细胞分裂和二氧化硅代谢有关。周生生物群落中的差异基因表达表明可能存在不同的周生生物分子表型，包括 D. geminata，与低磷位点的磷获取和多糖代谢以及高磷位点的细胞分裂和生长有关。172 个基因仅在非垫形成样品中表达，许多基因与细胞分裂和二氧化硅代谢有关。周生生物群落中的差异基因表达表明可能存在不同的周生生物分子表型，包括 D. geminata，与低磷位点的磷获取和多糖代谢以及高磷位点的细胞分裂和生长有关。